Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Next-generation Sequencing03:00

Next-generation Sequencing

98.0K
The first human genome sequencing project cost $2.7 billion and was declared complete in 2003, after 15 years of international cooperation and collaboration between several research teams and funding agencies. Today, with the advent of next-generation sequencing technologies, the cost and time of sequencing a human genome have dropped over 100 fold.
Next-Generation Sequencing Methods
Although all next-generation methods use different technologies, they all share a set of standard features....
98.0K
Clinical Applications of Epidermal Stem Cells01:19

Clinical Applications of Epidermal Stem Cells

3.3K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
3.3K
Local Anesthetics: Clinical Application as Spinal Anesthesia01:11

Local Anesthetics: Clinical Application as Spinal Anesthesia

1.4K
Spinal anesthetics are given during lower abdomen and limb surgeries to block sensory and motor neurons. They are administered in the mid to low lumbar regions, primarily acting on the cauda equina's nerve roots. The blockade level depends on the local anesthetic (LA) concentration. Usually, low LA concentrations are sufficient to block sensory fibers, while only high LA concentrations block motor fibers. Other factors like injection volume and speed, the patient's posture, and the drug...
1.4K
Local Anesthetics: Clinical Application as Epidural Anesthesia01:29

Local Anesthetics: Clinical Application as Epidural Anesthesia

695
Epidural anesthetics are administered in the fat-filled epidural space, the outermost part of the spinal canal. This technique is commonly employed for pain management and anesthesia during lower abdomen and pelvis surgeries or labor and delivery.
Since epidural anesthetics can be infused through an epidural catheter, all types of drugs, including short-acting ones, can be administered. Chloroprocaine and lidocaine are examples of short and long-duration anesthetics, respectively. Bupivacaine...
695
Local Anesthetics: Clinical Application as Intravenous Regional Anesthesia01:16

Local Anesthetics: Clinical Application as Intravenous Regional Anesthesia

1.3K
Intravenous regional anesthesia or the Bier block technique is used to anesthetize a specific limb or extremity. It uses exsanguinated or blood-drained vessels to transport local anesthetics or LAs to the peripheral nerve trunks. Lidocaine without vasoconstrictors like epinephrine is most commonly used for this technique. Other drugs used are prilocaine, ropivacaine, and chloroprocaine. Bupivacaine is not recommended for this technique due to its high cardiac toxicity.
One of the advantages of...
1.3K
Local Anesthetics: Clinical Application as Surface, Infiltration, and Conduction Block Anesthesia01:30

Local Anesthetics: Clinical Application as Surface, Infiltration, and Conduction Block Anesthesia

1.9K
Depending on the target organ, local anesthetics (LAs) can be administered via various routes. In surface anesthesia, LAs are applied directly to the surface of the skin or mucous membranes. It is widely used for topical skin numbing before venipuncture or minor surgical procedures. Commonly used surface local anesthetics are lidocaine or benzocaine sprays or creams. Surface anesthesia occurs within 5 minutes and lasts for about 60 minutes. One of the main disadvantages of topical anesthesia is...
1.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The effect of a proprietary chlorine-stabilizing surface-coating formulation on healthcare environment disinfection in multidrug-resistant bacteria isolation rooms.

Antimicrobial resistance and infection control·2026
Same author

Metagenomic analysis of antimicrobial resistance genes in domestic canines.

One health (Amsterdam, Netherlands)·2026
Same author

Premise plumbing bacterial communities in four European cities and their association with <i>Legionella</i>.

Frontiers in microbiomes·2026
Same author

Cat scratch disease encephalitis: New insights into rate, clinical features, and long-term outcomes.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2026
Same author

Detection of Paraburkholderia in Clinical Specimens Associated with Use of Nonsterile Ultrasound Gel for Percutaneous Procedures - United States, Canada, and Israel, May 2023⎯April 2025.

MMWR. Morbidity and mortality weekly report·2025
Same author

Trends in incidence and epidemiological characteristics of campylobacteriosis, Israel, 2013 to 2022.

Euro surveillance : bulletin Europeen sur les maladies transmissibles = European communicable disease bulletin·2025

Related Experiment Video

Updated: Jan 24, 2026

The Clinical Application of Tumor Treating Fields Therapy in Glioblastoma
08:00

The Clinical Application of Tumor Treating Fields Therapy in Glioblastoma

Published on: April 16, 2019

19.2K

Next-generation sequencing applications in clinical bacteriology.

Yair Motro1, Jacob Moran-Gilad1,2,3

  • 1Department of Health System Management, Faculty of Health Sciences, Ben-Gurion University of the Negev, Beer-Sheva, Israel.

Biomolecular Detection and Quantification
|December 20, 2017
PubMed
Summary

Next generation sequencing (NGS) is transforming clinical microbiology diagnostics. This review covers NGS application in labs, focusing on its impact on diagnostic stages.

Keywords:
ApplicationsClinical diagnosticsClinical microbiologyNext generation sequencingPublic healthWorkflow

More Related Videos

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
09:36

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

17.6K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.0K

Related Experiment Videos

Last Updated: Jan 24, 2026

The Clinical Application of Tumor Treating Fields Therapy in Glioblastoma
08:00

The Clinical Application of Tumor Treating Fields Therapy in Glioblastoma

Published on: April 16, 2019

19.2K
Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
09:36

Unbiased Deep Sequencing of RNA Viruses from Clinical Samples

Published on: July 2, 2016

17.6K
Targeted DNA Methylation Analysis by Next-generation Sequencing
08:38

Targeted DNA Methylation Analysis by Next-generation Sequencing

Published on: February 24, 2015

38.0K

Area of Science:

  • Clinical Microbiology
  • Molecular Diagnostics
  • Bacteriology

Background:

  • Next-generation sequencing (NGS) technologies are rapidly advancing.
  • Clinical and public health microbiology laboratories are increasingly adopting NGS.
  • NGS adoption impacts existing diagnostic workflows.

Purpose of the Study:

  • To review the application of NGS in clinical microbiology settings.
  • To highlight considerations for implementing NGS in diagnostic cycles.
  • To examine the impact of NGS on analytical and post-analytical diagnosis.

Main Methods:

  • Literature review focused on bacteriology.
  • Analysis of NGS implementation in clinical microbiology.
  • Evaluation of impacts on diagnostic stages.

Main Results:

  • NGS offers significant potential for clinical microbiology.
  • Implementation requires careful consideration of analytical and post-analytical processes.
  • Impacts span workflow integration and diagnostic output.

Conclusions:

  • NGS adoption is a key trend in modern clinical microbiology.
  • Successful implementation hinges on addressing specific analytical and post-analytical challenges.
  • NGS enhances diagnostic capabilities in bacteriology.