Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bone marrow-derived mononuclear phagocytes autoregulate mannose receptor expression.

D R Clohisy1, J C Chappel, S L Teitelbaum

  • 1Department of Pathology and Laboratory Medicine, Jewish Hospital at Washington University Medical Center, St. Louis, Missouri 63110.

The Journal of Biological Chemistry
|April 5, 1989
PubMed
Summary

Bone marrow macrophages increase mannose receptor expression by removing a serum factor that inhibits binding. This regulation is density-dependent and involves inactivating a noncompetitive, inhibitory protein in the culture medium.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Phthalate exposure and pubertal development in a longitudinal study of US girls.

Human reproduction (Oxford, England)·2014
Same author

The association between parental perception of neighborhood safety and asthma diagnosis in ethnic minority urban children.

Journal of urban health : bulletin of the New York Academy of Medicine·2012
Same author

Temporal variability in urinary concentrations of phthalate metabolites, phytoestrogens and phenols among minority children in the United States.

Environmental research·2007
Same author

Interactions among GSTM1, GSTT1 and GSTP1 polymorphisms, cruciferous vegetable intake and breast cancer risk.

Carcinogenesis·2007
Same author

Advances in understanding bone cancer pain.

Journal of cellular biochemistry·2005
Same author

Bone cancer pain and the role of RANKL/OPG.

Journal of musculoskeletal & neuronal interactions·2004

Area of Science:

  • Immunology
  • Cell Biology
  • Macrophage Biology

Background:

  • Surface mannose receptor expression on macrophages increases with culture time.
  • Previous studies suggested a time-dependent increase in mannose receptor expression.

Purpose of the Study:

  • To investigate the factors regulating mannose receptor expression in bone marrow-derived macrophages.
  • To determine if cell density or cell cycling influences mannose receptor expression.
  • To identify the mechanism behind density-dependent regulation of mannose receptor expression.

Main Methods:

  • Culturing macrophages at high and low densities.
  • Measuring 125I-mannose-bovine serum albumin (125I-Man-BSA) binding.
  • Using conditioned media from macrophage and control cultures.

Related Experiment Videos

  • Fractionating conditioned media to isolate inhibitory factors.
  • Characterizing the biochemical properties of the down-regulating factor.

    • Main Results:

    • Macrophage mannose receptor expression is density-dependent, not time-dependent.
    • High-density cultures show significantly higher 125I-Man-BSA binding per cell.
    • A down-regulating factor in culture medium, residing in fetal calf serum, inhibits mannose receptor expression.
    • The inhibitory factor is acid-activable, trypsin-sensitive, and heat-resistant.
    • Macrophage-conditioned medium lacks this inhibitory activity.

    Conclusions:

    • Bone marrow macrophages up-regulate mannose receptor expression by inactivating a noncompetitive, serum-residing inhibitory protein.
    • Cell density is a critical factor in regulating mannose receptor expression.
    • This mechanism involves macrophages actively removing an inhibitor from their environment.