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Related Experiment Videos

Plant cells contain calsequestrin.

K H Krause1, M Chou, M A Thomas

  • 1Department of Physiology and Biophysics, University of Iowa College of Medicine, Iowa City 52242.

The Journal of Biological Chemistry
|March 15, 1989
PubMed
Summary

Researchers discovered calsequestrin, a calcium-binding protein, in plant tissues like Streptanthus tortuosus cells and spinach leaves. This finding suggests calsequestrin is not exclusive to animal cells but is a ubiquitous protein.

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Area of Science:

  • Biochemistry
  • Cell Biology
  • Plant Science

Background:

  • Calsequestrin is a high-capacity, low-affinity Ca2+-binding protein crucial for intracellular calcium pools in animal cells.
  • Its role in plant cells, however, remained largely unexplored.

Purpose of the Study:

  • To investigate the presence and characteristics of calsequestrin in plant tissues.
  • To determine if calsequestrin is exclusive to animal cells or a more ubiquitous protein.

Main Methods:

  • Subcellular fractionation of cultured Streptanthus tortuosus cells.
  • Stains-all staining to identify the protein in microsomal fractions.
  • Biochemical characterization including pH-dependent molecular weight shifts and 45Ca2+ binding assays.
  • Immunological detection using antibodies against canine cardiac calsequestrin.

Related Experiment Videos

  • Direct extraction from spinach leaves using a muscle calsequestrin protocol.

    • Main Results:

    • A 56,000-Da protein with characteristics similar to animal calsequestrin was identified in S. tortuosus microsomal fractions.
    • This plant protein exhibited pH-dependent molecular weight changes and bound 45Ca2+.
    • The protein was recognized by antibodies specific to canine cardiac calsequestrin.
    • Calsequestrin was successfully extracted and identified in spinach leaves.

    Conclusions:

    • Plant cells, specifically S. tortuosus and spinach, contain a protein homologous to animal calsequestrin.
    • This discovery indicates that calsequestrin is not limited to animal cells but is likely a ubiquitous protein across different kingdoms.
    • The presence of calsequestrin in plants suggests a conserved role in intracellular calcium regulation.