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Related Experiment Video

Updated: Feb 16, 2026

Author Spotlight: Streamlining Rice Breeding with CRISPR/Cas for Obtaining Optimal Phenotypic and Agronomic Traits
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Genome Editing Tools in Plants.

Tapan Kumar Mohanta1, Tufail Bashir2, Abeer Hashem3,4

  • 1Department of Biotechnology, Yeungnam University, Gyeongsan 38541, Korea. nostoc.tapan@gmail.com.

Genes
|December 20, 2017
PubMed
Summary

Genome editing tools precisely alter genomic architecture for crop improvement. New adenine base editors (ABEs) offer precise A-T to G-C mutations, advancing plant genetic engineering.

Keywords:
CRISPR/Cas9RNAiTALENZinc finger nucleaseadenine base editorscisgenesis and intragenesisgenome editinghomologous recombinationoligonucleotide-directed mutagenesispentatricopeptide repeat proteinplastid genomesite-directed sequence editingsynthetic genomics

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Area of Science:

  • Plant genetics and genomics
  • Molecular biology
  • Biotechnology

Background:

  • Genome editing tools precisely modify DNA sequences.
  • These technologies are crucial for trait discovery and developing crops with enhanced yield and stress resistance.
  • Complex plant genomes present challenges for comprehensive gene editing.

Purpose of the Study:

  • To review major genome editing tools used in plants.
  • To highlight advancements in precise genome modification techniques.
  • To introduce adenine base editors (ABEs) for specific base pair conversions.

Main Methods:

  • Review of established genome editing technologies including Homologous recombination (HR), zinc finger nucleases (ZFNs), transcription activator-like effector nucleases (TALENs), pentatricopeptide repeat proteins (PPRs), CRISPR/Cas9, RNA interference (RNAi), cisgenesis, and intragenesis.
  • Discussion of site-directed sequence editing and oligonucleotide-directed mutagenesis for single-nucleotide alterations.
  • Description of adenine base editors (ABEs) utilizing deoxyadeninedeaminase (TadA) and catalytically impaired Cas9 nickase.

Main Results:

  • A diverse array of genome editing tools are available for plant genetic engineering.
  • CRISPR/Cas9, ZFNs, TALENs, and other methods enable targeted modifications.
  • Adenine base editors (ABEs) provide a novel method for specific A-T to G-C base pair conversions.

Conclusions:

  • Genome editing technologies are vital for advancing plant science and agriculture.
  • The development of precise tools like ABEs expands the capabilities for targeted genetic improvement in plants.
  • Continued innovation in genome editing promises significant contributions to crop development.