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STEM CELLS HEMATOPOIETIC NICHES AND INFLAMMATORY RESPONSE TO DIFFERENT SYNTHETIC PROSTHESIS IMPLANTED IN RAT WITH
Renato Lamounier Barbieri1, Suely de Fátima Parreira1, Sarah do Valle Studart1
1Medical School, Nove de Julho University, São Paulo, SP, Brazil.
Summary
Incisional hernia repair with polypropylene prostheses induced significant inflammatory responses and megakaryocyte niches in rats. Further studies are needed to understand these findings related to extramedullary hematopoiesis.
Area of Science:
- Surgical Science
- Pathophysiology
- Biomaterials Science
Background:
- Extramedullary hematopoiesis involves hematopoietic stem cells and inflammation mediators.
- Identifying hematopoietic cells outside bone marrow was once considered secondary but is now recognized as significant.
- Biomolecular studies have reshaped the understanding of extramedullary hematopoiesis.
Purpose of the Study:
- To investigate the presence of megakaryocytes and inflammatory cells in rat abdominal walls and spleens after incisional hernia repair with various synthetic prostheses.
- To analyze the impact of different polypropylene-based prostheses on cellular responses in hernia defects.
Main Methods:
- Twenty-five rats with incisional hernias were divided into five groups, undergoing repair with different prosthetic materials or no implant.
- Prostheses were rhombus-shaped (2.625 cm²).
- Animals were reoperated after 10 days, with abdominal walls and attached viscera collected for histological analysis.
Main Results:
- Megakaryocyte niches were observed in the abdominal wall and spleen.
- These niches were found alongside adhesions in animals with implanted prostheses.
- A significant inflammatory reaction was noted in conjunction with the prostheses.
Conclusions:
- The intense inflammatory reaction associated with polypropylene prostheses was disproportionate to expectations.
- Further research, including immunophenotyping and specific monoclonal antibody panels, is recommended.
- These studies are crucial for a comprehensive understanding of the observed cellular responses.
