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Related Concept Videos

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

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Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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FISH - Fluorescent In-situ Hybridization02:07

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Fluorescence in situ hybridization, or FISH, was developed in the early 1980s and has quickly become one of the most widely used techniques in cytogenetics. Labeled probes are used to bind complementary DNA or RNA sequences on a chromosome or in a region within a cell. Earlier, the probes could only be obtained by cloning or reverse transcription of a DNA template. Currently, the probe oligonucleotides can be synthesized synthetically. Additionally, with the advancement of optical techniques,...
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Gene Duplication and Divergence02:37

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The seminal work of Ohno in 1970 popularized the idea of gene duplication and divergence. DNA sequence comparison studies reveal that a large portion of the genes in bacteria, archaebacteria, and eukaryotes was  generated by gene duplication and divergence, indicating its critical role in evolution.
The duplicated copies of the gene are called Paralogs. Paralogs with similar sequences and functions form a gene family. Across several species, a large number of gene families are...
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In-situ Hybridization02:31

In-situ Hybridization

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In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
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¹H NMR Signal Integration: Overview00:58

¹H NMR Signal Integration: Overview

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The intensity of a signal, which can be represented by the area under the peak, depends on the number of protons contributing to that signal. The area under each peak is shown as a vertical line called an integral, with the integral value listed under it, as seen in the proton NMR spectrum of benzyl acetate. Each integral value is divided by the smallest integral value to obtain the ratio of the number of protons producing each signal. The ratio reveals the relative number of protons and not...
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Combinatorial Gene Control02:33

Combinatorial Gene Control

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Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
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Related Experiment Video

Updated: Feb 16, 2026

Oncogenic Gene Fusion Detection Using Anchored Multiplex Polymerase Chain Reaction Followed by Next Generation Sequencing
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INTEGRATE-Vis: a tool for comprehensive gene fusion visualization.

Jin Zhang1, Teng Gao2,3, Christopher A Maher4,5,6,7

  • 1Department of Radiation Oncology, Washington University School of Medicine, St. Louis, Missouri, 63110, USA.

Scientific Reports
|December 21, 2017
PubMed
Summary
This summary is machine-generated.

Predicting gene fusions from sequencing data is challenging. INTEGRATE-Vis automates gene fusion annotation and visualization, aiding in understanding novel protein products and gene expression changes.

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Area of Science:

  • Genomics
  • Bioinformatics
  • Molecular Biology

Background:

  • Accurate prediction of gene fusions from sequencing data is crucial for understanding their biological consequences.
  • Current methods rely on manual inspection, which is labor-intensive and time-consuming.
  • Automating the annotation and visualization of gene fusions can streamline this process.

Purpose of the Study:

  • To develop a user-friendly tool for visualizing gene fusion products.
  • To automate the annotation of gene fusions at transcript and protein levels.
  • To assess gene fusion expression within individual samples and patient cohorts.

Main Methods:

  • Development of INTEGRATE-Vis, a gene fusion visualization software.
  • Generation of customizable, publication-quality graphics.
  • Focus on annotating transcript- and protein-level fusion events and assessing expression.

Main Results:

  • INTEGRATE-Vis provides comprehensive visualizations of gene fusion products.
  • The tool facilitates inference of biological consequences, such as novel proteins and altered gene expression.
  • It supports analysis across individual samples and patient cohorts.

Conclusions:

  • INTEGRATE-Vis is the first comprehensive tool for visualizing gene fusions and inferring their consequences.
  • The software aids in understanding the biological impact of gene fusion events.
  • INTEGRATE-Vis has potential applications in both research and clinical settings.