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Fluorimetric Techniques for the Assessment of Sperm Membranes
Published on: November 28, 2018
Protein biomarkers for male artificial insemination subfertility in bovine spermatozoa
Hiroshi Harayama1, Kenta Minami1, Kazumi Kishida2
1Division of Animal Science Department of Bioresource Science Graduate School of Agricultural Science Kobe University Kobe Japan.
Background:
Although artificial insemination (AI) technique is an established biotechnology for bovine reproduction, the results of AI (conception rates) have a tendency to decline gradually. To our annoyance, moreover, AI-subfertile bulls have been occasionally found in the AI centers. To resolve these serious problems, it is necessary to control the sperm quality more strictly by the examinations of sperm molecules.
Methods:
We reviewed a number of recent articles regarding potentials of bovine sperm proteins as the biomarkers for bull AI-subfertility and also showed our unpublished supplemental data on the bull AI-subfertility associated proteins.
Main Findings:
Bull AI-subfertility is caused by the deficiency or dysfunctions of various molecules including regulatory proteins of ATP synthesis, acrosomal proteins, nuclear proteins, capacitation-related proteins and seminal plasma proteins.
Conclusion:
In order to control the bovine sperm quality more strictly by the molecular examinations, it is necessary to select suitable sperm protein biomarkers for the male reproductive problems which happen in the AI centers.
