Related Experiment Video

Updated: Feb 16, 2026

Isolation of CD133+ Liver Stem Cells for Clonal Expansion
12:06

Isolation of CD133+ Liver Stem Cells for Clonal Expansion

Published on: October 10, 2011

22.9K

Cancer cell reprogramming to identify the genes competent for generating liver cancer stem cells

Kenly Wuputra1, Chang-Shen Lin1,2, Ming-Ho Tsai1

  • 1Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, 807 Taiwan.

Inflammation and Regeneration
|December 21, 2017
PubMed

Insights

Cancer stem cells (CSCs) drive liver cancer progression and drug resistance. Induced pluripotent stem cell (iPSC) technology offers a new way to study liver CSCs and develop targeted cancer therapies.

Area of Science:

  • Oncology
  • Stem Cell Biology
  • Cancer Research

Background:

  • The cancer stem cell (CSC) hypothesis suggests that cancers, including liver cancer, originate from transformed stem/progenitor cells.
  • CSCs are implicated in cancer's drug resistance, metastasis, and recurrence, making them critical therapeutic targets.

Purpose of the Study:

  • To introduce a novel method for identifying potential liver cancer stem cell (CSC) candidates.
  • To explore the utility of induced pluripotent stem cell (iPSC) technology for studying CSCs and developing new cancer treatments.

Main Methods:

  • Utilizing induced pluripotent stem cell (iPSC) technology to reprogram cancer cells.
  • Investigating the interactions between CSC-related genes and the tumor microenvironment.
  • Analyzing transcription factors involved in generating liver CSCs from iPSCs.

Main Results:

  • iPSC technology provides a valuable platform for studying CSC generation and cancer initiation mechanisms.
  • The reprogramming process allows for the examination of CSC-related genes before and after iPSC generation.
  • This approach facilitates the study of liver CSCs and their role in oncogenesis.

Conclusions:

  • Targeting CSCs is essential for effective liver cancer treatment.
  • iPSC technology is a promising tool for understanding liver CSCs and developing novel therapeutic strategies.
  • Further research into transcription factors and microenvironmental interactions is crucial for modeling liver cancer development.
Keywords:
Induced pluripotent stem cellsLiver cancerOCT4Reprogrammingc-JUN oncogene

More Related Videos

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets07:08

Author Spotlight: Reprogramming Cancer Cells to iPSCs to Study Disease Progression and Treatment Targets

Published on: February 2, 2024

1.5K
Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres06:52

Discovery of Driver Genes in Colorectal HT29-derived Cancer Stem-Like Tumorspheres

Published on: July 22, 2020

7.0K

Related Concept Videos

Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
6.1K
Somatic to iPS Cell Reprogramming01:29

Somatic to iPS Cell Reprogramming

Reprogramming alters the gene expression in somatic cells, transforming them into induced pluripotent stem (iPS) cells over several generations. Scientists can reprogram cells by introducing genes for four transcription factors—Oct4, Sox2, Klf4, and c-Myc (OSKM) by viral or non-viral methods. These factors are also known as Yamanaka factors after Shinya Yamanaka, who first generated iPS cells using mouse skin cells. Yamanaka was awarded the Nobel Prize in Physiology or Medicine in 2012...
2.7K
Liver Regeneration01:24

Liver Regeneration

The liver is an important organ in vertebrates that plays an essential role in metabolism. It is also responsible for storing and redistributing nutrients such as carbohydrates, fats, and vitamins in the body. Additionally, the liver releases bile salts which are critical for digesting food and eliminating toxic metabolites from the body.
Cells of Liver
The liver comprises four major types of cells— hepatocytes, stellate, Kupffer, and sinusoidal endothelial cells. The hepatocytes are...
4.5K
Methods of Nuclear Reprogramming01:24

Methods of Nuclear Reprogramming

Nuclear reprogramming is a process of transforming one cell type into an unrelated cell type by epigenetic changes that alter the cell’s original gene expression pattern. Such epigenetic changes force cells to express a different set of genes, which play a significant role in inducing transformation into other cell types. Nuclear reprogramming offers applications in reproductive cloning for livestock propagation and regenerative medicine — developing patient-specific cells for...
2.2K