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Updated: Feb 16, 2026
Induction of Adhesion-dependent Signals Using Low-intensity Ultrasound
Published on: May 8, 2012
[Protective Effect and Its Possible Mechanism of Ulinastatin on Lung Injury Induced by Hip Fracture]
Xiao-Dan Zhao1, Hao Liu1, Tao Li1
1Department of Orthopedics,West China Hospital,Sichuan University,Chengdu 610041,China.
Objective:
To investigate the protective effect and its possible mechanism of ulinastatin on lung injury caused by hip fracture.
Methods:
Total 24 SD rats were randomly divided into control,hip fracture and hip fracture+ulinastatin groups (n=8). The rats of hip fracture and hip fracture+ulinastatin groups made the left hip fracture. The tail vein of rats of hip fracture+ulinastatin group were injected with 50 000 U/kg ustodin before made fracture model. Killed rats 24 h after mold building. Serum mtDNA,tumor necrosis factor-α (TNF-α) and interleukin (IL)-6 in bronchoalveolar lavage fluid,Toll like receptor-9 (TLR9) and nuclear factor (NF)-κB protein expression in lung tissue,wet/dry ratio and pathological scores of lung were detected in all three groups.
Results:
Serum mtDNA,TNF-α and IL-6 in bronchoalveolar lavage fluid,TLR9 and NF-κB protein expressions in lung tissue,wet/dry ratio and pathological scores of lung in hip fracture group were significantly higher than those in control group ( P<0.05). Ulinastatin significantly reduced the levels of serum mtDNA,TNF-α and IL-6 in bronchoalveolar lavage fluid,TLR9 and NF-κB protein expressions in lung tissue,as well as wet/dry ratio and pathological scores of lung ( P<0.05).
Conclusion:
Ulinastatin might could provide protection on lung injury in hip fracture,and mtDNA-TLR9-NF-κB pathway may play the role of reducing inflammatory level in the process.
