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Published on: July 3, 2013
[Expression and Significance of PI-PLCε1 in Colon Cancer]
Xiao-Ran Li1, Kun Yang1, Xiao-Li Huang1
1Department of Geriatrics Medicine,West China Hospital,Sichuan University,Chengdu 610041,China.
Objective:
To study the expression and clinical significance of phosphoinositide-specific phospholipase Cε1 (PI-PLCε1) in the pathogenesis of colon cancer.
Methods:
qRT-PCR and immunohistochemistry were used to detect the expression of PI-PLCε1in the 42 cases of colon cancer tissues and their corresponding adjacent tissues. And the effects of tumor differentiation and tumor site on the expression PI-PLCε1 of colon cancer tissues were compared.
Results:
The results of qRT-PCR showed that the expression of PI-PLCε1in colon cancer tissue significantly lower than that in the adjacent tissue ( P<0.05). The expression of PI-PLCε1gene of colon cancer tissue was not effected by tumor differentiation and tumor site ( P>0.05). The results of immuno-histochemistry showed that the positive expression rate of PI-PLCε1 protein in colon cancer tissue was significantly lower than that in the adjacent tissue ( P<0.05). The positive expression rate of PI-PLCε1 protein was not effected by tumor differentiation ( P>0.05),but the expression was different in tumor site ( P<0.05).
Conclusion:
Expression of PI-PLCε1 was reduced in colon tissue and barely to tumor differentiation.
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