Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Synaptic protein mutations in autism.

Neurobiology of disease·2026
Same author

Healthcare costs and health outcomes analysis of neoadjuvant Trastuzumab therapy for human epidermal growth factor receptor 2 (HER2) positive breast cancer.

Cancer pathogenesis and therapy·2026
Same author

Correction: Assessing the clinical utility of pre-operative neutrophil-lymphocyte ratio as a predictor of clinicopathological parameters in patients being treated for primary breast cancer.

Breast cancer research and treatment·2025
Same author

Assessing the clinical utility of pre-operative neutrophil-lymphocyte ratio as a predictor of clinicopathological parameters in patients being treated for primary breast cancer.

Breast cancer research and treatment·2025
Same author

Evaluating the Cellular Roles of the Lysine Acetyltransferase Tip60 in Cancer: A Multi-Action Molecular Target for Precision Oncology.

Cancers·2024
Same author

Centrosome amplification promotes cell invasion via cell-cell contact disruption and Rap-1 activation.

Journal of cell science·2023

Related Experiment Video

Updated: Feb 16, 2026

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

7.0K

In Vitro Histone Acetylation Assay.

James A L Brown1

  • 1Discipline of Surgery, Lambe Institute for Translational Research, School of Medicine, National University of Ireland, Galway, Ireland.

Current Protocols in Pharmacology
|December 21, 2017
PubMed
Summary

Histone acetyltransferases (HATs) are crucial enzymes regulating gene expression and metabolism. This study details an in vitro assay for quantifying HAT activity, essential for understanding acetylation

Keywords:
HATacetylationacetyltransferaseanalysisassayhistonein vitroquantitative

More Related Videos

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
05:44

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

1.4K
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
09:07

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Published on: May 1, 2019

7.3K

Related Experiment Videos

Last Updated: Feb 16, 2026

Assays for Validating Histone Acetyltransferase Inhibitors
09:11

Assays for Validating Histone Acetyltransferase Inhibitors

Published on: August 6, 2020

7.0K
Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening
05:44

Author Spotlight: Developing Acetyl-Click Assay for HAT1 Inhibitor Screening

Published on: January 26, 2024

1.4K
Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay
09:07

Single-Step Enrichment of a TAP-Tagged Histone Deacetylase of the Filamentous Fungus Aspergillus nidulans for Enzymatic Activity Assay

Published on: May 1, 2019

7.3K

Area of Science:

  • Biochemistry
  • Molecular Biology
  • Epigenetics

Background:

  • Acetylation is a fundamental cellular process impacting genomic stability, gene regulation, and metabolism.
  • Histone acetyltransferases (HATs) catalyze acetylation, making them key regulators of these cellular functions.
  • Dysregulated acetylation is linked to various diseases, notably cancer, highlighting the importance of HATs.

Purpose of the Study:

  • To detail a reliable in vitro acetylation assay for quantifying histone acetyltransferase (HAT) activity.
  • To provide a method for assessing HAT enzyme function, crucial for understanding cellular acetylation.

Main Methods:

  • Description of an in vitro acetylation assay protocol.
  • Utilizes either endogenous or tagged overexpressed proteins for HAT activity measurement.
  • Focuses on quantifying the catalytic activity of HAT enzymes.

Main Results:

  • The study provides a detailed methodology for an in vitro HAT acetylation assay.
  • The assay allows for the quantification of enzyme activity.

Conclusions:

  • In vitro histone acetylation assays are vital tools for studying HAT activity.
  • This assay facilitates research into the role of HATs in normal cellular processes and disease states.