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Detection of microRNA Expression in Peritoneal Membrane of Rats Using Quantitative Real-time PCR
Published on: June 27, 2017
Characteristic MicroRNA Expression Induced by δ-Opioid Receptor Activation in the Rat Liver Under Prolonged Hypoxia
Feng Zhi1,2, Naiyuan Shao1, Lian Xue2
1Department of Neurosurgery, The First People's Hospital of Changzhou, Changzhou, China.
Background/Aims:
Hypoxic/ischemic injury to the liver is a frequently encountered clinical problem with limited therapeutic options. Since microRNAs (miRNAs) are involved in hypoxic/ ischemic events, and δ-opioid receptor (DOR) is protective against hypoxic/ischemic injury, we asked if pharmacological activation of DOR can alter hypoxic events by regulating miRNA expression in the liver. As the first step, the present work aimed at testing the effect of DOR activation on hepatic miRNA expression in hypoxia.
Methods:
Male Sprague Dawley rats were exposed to hypoxia (9.5-10% O2) for 1, 5, or 10 days with or without DOR activation. The target miRNAs were selected according to TaqMan low-density array (TLDA) data and analyzed by quantitative real-time PCR.
Results:
We found that: 1) 1-day hypoxia caused the upregulation of 9 miRNAs (miR-7a-5p, miR-10a-5p, miR-25-3p, miR-26b-5p, miR-122-5p, miR-128a-3p, miR-135b-5p, miR-145-5p, and miR-181a-5p) and the downregulation of 2 miRNAs (miR-34a-5p and miR-182); 2) 5 and 10-days hypoxia altered the expression of 4 miRNAs (miR-34c-5p, miR-184, miR-107-3p and miR192-5p); 3) DOR activation shifted the expression of 8 miRNAs (miR-122-5p, miR-146a-5p, miR-30e-5p, miR-128a-3p, miR-182, miR-192-5p miR-107-3p and miR-184) in normoxic condition; and 4) DOR activation modified hypoxia-induced changes in 6 miRNAs (miR-142-5p, miR-145-5p, miR-146a-5p, miR-204-5p, miR-34a-5p and miR-192-5p).
Conclusion:
Hypoxia significantly modifies the miRNA profile in the liver, while DOR activation can modify the hypoxic modification. Therefore, it is potentially possible to alter hypoxic/ischemic pathophysiology in the liver through DOR pharmacotherapy.
Insights
Hypoxia alters liver microRNA (miRNA) profiles. Activating the delta-opioid receptor (DOR) can modify these hypoxia-induced changes, suggesting a potential therapeutic strategy for liver injury.
Area of Science:
- Hepatology
- Molecular Biology
- Pharmacology
Background:
- Hypoxic/ischemic liver injury presents a significant clinical challenge with limited treatment options.
- MicroRNAs (miRNAs) play a role in hypoxic/ischemic events.
- Delta-opioid receptor (DOR) activation is known to be protective against hypoxic/ischemic injury.
Purpose of the Study:
- To investigate the effect of delta-opioid receptor (DOR) activation on hepatic miRNA expression under hypoxic conditions.
- To determine if pharmacological activation of DOR can modulate miRNA expression during hypoxia in the liver.
- To explore potential therapeutic strategies for hypoxic liver injury by targeting DOR and miRNA pathways.
Main Methods:
- Male Sprague Dawley rats were subjected to hypoxia (9.5-10% O2) for 1, 5, or 10 days.
- DOR activation was administered with or without hypoxia exposure.
- miRNA expression was analyzed using TaqMan low-density array (TLDA) and quantitative real-time PCR.
Main Results:
- One-day hypoxia upregulated 9 miRNAs and downregulated 2 miRNAs.
- Prolonged hypoxia (5 and 10 days) altered the expression of 4 specific miRNAs.
- DOR activation alone shifted the expression of 8 miRNAs in normoxic conditions.
- DOR activation modulated hypoxia-induced changes in 6 key miRNAs.
Conclusions:
- Hypoxia significantly alters the miRNA expression profile in the liver.
- DOR activation demonstrates the capacity to modify hypoxia-induced changes in hepatic miRNA expression.
- Targeting DOR pharmacotherapy presents a potential avenue for altering hypoxic/ischemic pathophysiology in the liver.
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