Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Hepatocyte iron release in rats.

Y Beguin1, H A Huebers, G Weber

  • 1Department of Medicine, University of Washington, Seattle.

The Journal of Laboratory and Clinical Medicine
|March 1, 1989
PubMed
Summary

Hepatocyte iron release in rats showed that about 6% of storage iron is released daily. Iron overload creates a non-mobilizable pool, and inflammation blocks iron release, suggesting transferrin receptors regulate iron mobilization.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Partial marrow shielding and total-body irradiation

Journal of applied physiology (Bethesda, Md. : 1985)·2019
Same author

Clinical syndrome of potassium intoxication.

The American journal of medicine·2010
Same author

Methemoglobin reconversion in the erythrocyte.

The Journal of clinical investigation·2010
Same author

Idiopathic thrombocytopenic purpura in a woman with prolonged exposure to benzol.

The Journal of industrial hygiene and toxicology·2010
Same author

Changes in circulating leukocytes induced by pituitary adrenocorticotropic hormone (ACTH) in man.

The Journal of clinical endocrinology and metabolism·2010
Same author

The in vitro preservation and post-transfusion survival of stored blood.

The Journal of clinical investigation·2010

Area of Science:

  • Hepatocyte iron metabolism
  • In vivo animal studies
  • Iron homeostasis regulation

Background:

  • Hepatocytes play a central role in iron storage and release.
  • Understanding hepatocyte iron release is crucial for managing iron overload disorders.
  • Regulation of iron release from hepatocytes is not fully understood.

Purpose of the Study:

  • To investigate the dynamics of iron release from hepatocytes in vivo.
  • To identify factors influencing hepatocyte iron mobilization.
  • To explore the role of plasma transferrin receptors in iron release regulation.

Main Methods:

  • In vivo study using iron-59 labeled compounds (ferritin, hemoglobin, asialotransferrin) in rats.
  • Simultaneous injection of iron-55 labeled rat transferrin to quantify storage iron release.

Related Experiment Videos

  • Measurement of radioactive iron incorporation into red blood cells over 2 weeks.
  • Assessment of iron release under conditions of normal iron status, iron overload, inflammation, and varying erythropoiesis.

    • Main Results:

    • Different iron compounds are processed uniquely by hepatocytes but stored similarly.
    • Approximately 6% of hepatocyte storage iron is released daily in normal rats.
    • Iron overload identified a distinct pool of iron resistant to spontaneous mobilization.
    • Inflammation significantly inhibited hepatocyte iron release.
    • Hepatocyte iron release correlated strongly with plasma transferrin receptor levels (p < 0.001).

    Conclusions:

    • Hepatocyte iron turnover is influenced by erythropoiesis rate and animal's iron status.
    • Inflammation acts as a barrier to hepatocyte iron release.
    • Plasma transferrin receptors may act as mediators in regulating hepatocyte iron mobilization in rats.