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The roles of ING5 expression in ovarian carcinogenesis and subsequent progression: a target of gene therapy
Hua-Chuan Zheng1, Shuang Zhao1, Yang Song2
1Department of Experimental Oncology and Animal Center, Shengjing Hospital of China Medical University, Shenyang 110004, China.
Abstract:
Here, we found that ING5 overexpression suppressed cell viability, glucose metabolism, migration, invasion and epithelial-mesenchymal transition, and induced cell arrest, apoptosis, senescence, autophagy and fat accumulation in ovarian cancer cells. ING5-mediated chemoresistance was positively linked to apoptotic resistance and chemoresistance-related gene expression. ING5 overexpression suppressed tumor growth of ovarian cancer by decreasing proliferation, and inducing apoptosis and autophagy. ING5 mRNA level was lower in ovarian cancer than normal ovary, and borderline than benign tumors (p < 0.05), and negatively correlated with vascular invasion, lymphatic invasion and FIGO staging of ovarian cancer (p < 0.05). ING5 protein was less expressed in primary cancer than normal ovary (p < 0.05). There was a negative correlation between ING5 mRNA expression and the overall or progression-free survival time of the cancer patients with Grade 2, Grade 3, and stage I cancer (p < 0.05). Immunohistochemically, ING5 was less expressed in serous and mucinous adenocarcinoma than miscellaneous subtypes, and positively correlated with dedifferentiation and ki-67 expression of ovarian cancer (p < 0.05). These data suggested that down-regulated ING5 expression might be involved in ovarian carcinogenesis possibly by suppressing aggressive phenotypes, including proliferation, tumor growth, migration, invasion, and anti-apoptosis.
Insights
Inhibitor of Growth 5 (ING5) suppresses ovarian cancer progression and aggressiveness. Lower ING5 levels correlate with poor survival, suggesting ING5’s tumor-suppressive role in ovarian cancer.
Area of Science:
- Oncology
- Molecular Biology
- Biochemistry
Background:
- Ovarian cancer is a leading cause of cancer-related mortality.
- Understanding the molecular mechanisms underlying ovarian cancer progression is crucial for developing effective therapies.
Purpose of the Study:
- To investigate the role of ING5 in ovarian cancer.
- To determine the correlation between ING5 expression and clinicopathological features and patient survival.
Main Methods:
- In vitro studies using ovarian cancer cell lines to assess the effects of ING5 overexpression on cell behavior.
- In vivo studies to evaluate the impact of ING5 on tumor growth.
- Analysis of ING5 mRNA and protein expression in patient tumor samples.
- Correlation analysis between ING5 expression and clinicopathological parameters, including survival data.
Main Results:
- ING5 overexpression suppressed ovarian cancer cell viability, migration, invasion, and epithelial-mesenchymal transition, while inducing apoptosis and autophagy.
- ING5 overexpression inhibited tumor growth in vivo.
- Lower ING5 mRNA and protein levels were observed in ovarian cancer tissues compared to normal ovarian tissues.
- Reduced ING5 expression correlated with advanced FIGO stage, lymphovascular invasion, and poorer patient survival.
- ING5 expression was negatively correlated with aggressive subtypes and positively with dedifferentiation and Ki-67 expression.
Conclusions:
- Down-regulation of ING5 is implicated in ovarian carcinogenesis.
- ING5 acts as a tumor suppressor by inhibiting aggressive phenotypes in ovarian cancer.
- ING5 may serve as a potential prognostic biomarker for ovarian cancer.
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