Related Experiment Video
Updated: Feb 16, 2026
Assessing Therapeutic Angiogenesis in a Murine Model of Hindlimb Ischemia
Published on: June 8, 2019
Evaluation of Chitotriosidase as a Marker of Inflammatory Status in Critical Limb Ischemia
Răzvan A Ciocan1,2, Cristina Drugan3, Claudia D Gherman2
1Department of Medical Informatics and Biostatistics, "Iuliu Haţieganu" University of Medicine and Pharmacy, Cluj-Napoca, Romania.
Insights
Elevated chitotriosidase activity in critical limb ischemia (CLI) patients suggests its potential as an inflammatory marker. However, smoking significantly influences these levels, requiring careful interpretation in CLI monitoring.
Area of Science:
- Biochemistry
- Immunology
- Vascular Medicine
Background:
- Chitotriosidase is an enzyme released by activated macrophages.
- Its role as a marker for inflammatory conditions is under investigation.
Purpose of the Study:
- To evaluate circulating chitotriosidase activity as a marker of inflammation in critical limb ischemia (CLI).
Main Methods:
- An observational, gender-matched case-control study.
- Included patients diagnosed with CLI and healthy volunteers.
- Measured chitotriosidase activity and analyzed diagnostic accuracy using ROC analysis.
Main Results:
- CLI patients exhibited significantly higher chitotriosidase activity compared to controls (p<0.001).
- Smokers showed elevated activity in both groups, with higher levels in CLI patients.
- ROC analysis assessed diagnostic accuracy for CLI.
Conclusions:
- Circulating chitotriosidase shows potential for monitoring CLI patients without other inflammatory diseases.
- Elevated levels necessitate consideration of tobacco-induced inflammation for accurate interpretation.
Background:
Chitotriosidase is an enzyme secreted by activated macrophages. This study aims to investigate the usefulness of circulating chitotriosidase activity as a marker of inflammatory status in patients with critical limb ischemia (CLI).
Materials And Methods:
An observational gender-matched case-control study was conducted on patients hospitalized with the primary diagnosis of CLI, as well as a control group. The control group consisted of healthy volunteers.
Results:
Forty-three patients were included in each group. Similar demographic characteristics (median age of 60-62 years and overweight) were observed in both groups. Chitotriosidase activity ranged from 110 nmol/ml/hr to 1530 nmol/ml/hr in the CLI group and from 30 nmol/ml/hr to 440 nmol/ml/hr in the control group; demonstrating significantly elevated values in the CLI group (p<0.001). Median plasma chitotriosidase activity was significantly elevated in smokers compared with non-smokers in both groups (p<0.05). However, this activity had higher values in CLI than in control subjects. Receiver operating characteristic (ROC) analysis was then performed in order to verify the diagnostic accuracy of chitotriosidase as an inflammatory biomarker in CLI.
Conclusion:
Circulating chitotriosidase is a test which can potentially be used for the monitoring of CLI patients without other inflammatory conditions. However, the interpretation of elevated values must take into account the inflammatory response induced by tobacco exposure.
More Related Videos
07:25Predicting Amputation using Local Circulating Mononuclear Progenitor Cells in Angioplasty-treated Patients with Critical Limb Ischemia
Published on: September 22, 2020
10:03Coronary Progenitor Cells and Soluble Biomarkers in Cardiovascular Prognosis after Coronary Angioplasty
Published on: January 28, 2020
Related Concept Videos
01:30Acute Coronary Syndrome III: Diagnostic Studies
01:20Blood Studies for Cardiovascular System I: Cardiac Biomarkers
The essential diagnostic tools for detecting myocardial necrosis and monitoring individuals suspected of having acute coronary syndrome (ACS) include:
Troponins
Troponins, particularly cardiac troponins I and T, are the most precise and sensitive markers of myocardial injury. They are detectable within 4-6 hours of myocardial injury and remain...