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Identification and Characterization of Memecylon Species Using Isozyme Profiling.

T R Bharathi1, Shailasree Sekhar2, N Geetha1

  • 1Department of Studies in Biotechnology, University of Mysore, Mysore, India.

Pharmacognosy Research
|December 22, 2017
PubMed
Summary

Protein and isozyme fingerprints are effective biochemical markers for identifying five Memecylon species. This study used polyacrylamide gel electrophoresis (PAGE) to reveal genetic similarities aligning with morphological classifications.

Keywords:
Acid phosphataseesterasenative polyacrylamide gel electrophoresisperoxidasepolyphenol oxidasesodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Area of Science:

  • Biochemistry
  • Plant Systematics
  • Molecular Biology

Background:

  • Protein and isozyme fingerprints serve as crucial biochemical markers for species identification and systematic studies in medicinal plants.
  • Differentiating plant species using biochemical profiles aids in understanding their genetic relationships and taxonomic classifications.

Purpose of the Study:

  • To investigate and compare the protein and isozyme profiles of five distinct Memecylon species: M. umbellatum, M. edule, M. talbotianum, M. malabaricum, and M. wightii.
  • To utilize biochemical characterization as a tool for accurate identification and systematic classification within the Memecylon genus.

Main Methods:

  • Enzyme extraction from fresh leaves of five Memecylon species.
  • Analysis of isozyme variations for peroxidase, esterase, acid phosphatase, polyphenol oxidase, alcohol dehydrogenase, and alkaline phosphatase using polyacrylamide gel electrophoresis (PAGE).
  • Construction of dendrograms based on similarity coefficients derived from protein banding patterns to assess genetic relationships.

Main Results:

  • Polyacrylamide gel electrophoresis (PAGE) analysis yielded a total of 50 bands across the five Memecylon species.
  • M. wightii exhibited the highest number of bands, while M. edule showed the fewest.
  • Similarity indices clustered M. malabaricum and M. wightii together (98% similarity) and M. umbellatum, M. edule, and M. talbotianum in a separate group (79% similarity), supporting morphological classifications.

Conclusions:

  • Protein and isozyme profiling provide a reliable biochemical fingerprint for differentiating and identifying Memecylon species.
  • The observed genetic similarities derived from isozyme analysis are consistent with morphological identifications, validating the biochemical approach.
  • This study represents the first comparative analysis of protein and isozyme profiles across these five Memecylon species.