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Upright Imaging of Drosophila Egg Chambers
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Two Drosophilids exhibit distinct EGF pathway patterns in oogenesis.

Kenley N O'Hanlon1, Rachel A Dam2, Sophie L Archambeault2,3

  • 1Department of Genome Sciences, University of Washington, 3720 15th AVE NE, Seattle, WA, 98195-5065, USA.

Development Genes and Evolution
|December 22, 2017
PubMed
Summary

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Investigating dorsal appendage development in fruit flies reveals differences in EGF signaling pathways, impacting tube-forming mechanisms and evolutionary changes in respiratory structures.

Area of Science:

  • Developmental biology
  • Evolutionary developmental biology
  • Insect morphology

Background:

  • Dorsal appendages in insect eggshells are key for studying morphological evolution.
  • Drosophilid species exhibit significant variation in appendage number and form.
  • Understanding patterning differences can explain morphogenetic variations.

Purpose of the Study:

  • To investigate the patterning differences underlying morphogenetic variations in insect dorsal appendages.
  • To compare EGF signaling in Drosophila melanogaster and Scaptodrosophila lebanonensis.
  • To elucidate how regulatory changes in patterning genes contribute to tube formation.

Main Methods:

  • Comparative analysis of BMP and EGF signaling pathways.
  • Gene expression analysis of key pathway components (thickveins, P-Mad, gurken, MAP kinase, broad, pointed, argos, Capicua).
Keywords:
Dorsal appendagesDrosophilaEggshellEpithelial morphogenesisFollicle cellsScaptodrosophila

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  • Functional assays using gene homologs between species.

    • Main Results:

    • Anterior patterning via BMP signaling is conserved between D. melanogaster and S. lebanonensis.
    • EGF signaling shows significant differences, with gurken forming a single primordium in S. lebanonensis.
    • Expression patterns of EGF pathway genes exhibit a heterochronic shift in S. lebanonensis.
    • The S. lebanonensis Gurken homolog is insufficient to alter D. melanogaster patterning.

    Conclusions:

    • EGF signaling pathway regulation differs significantly between D. melanogaster and S. lebanonensis.
    • Changes in EGF signaling patterns contribute to distinct tube-forming mechanisms.
    • This study provides insights into the genetic basis of morphological evolution in insect respiratory structures.