Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Autoreactive antibody production by intrarenal B cells in mouse kidney allograft rejection.

bioRxiv : the preprint server for biology·2026
Same author

The Alpha-beta Soup of HLA-DQ: A Recipe for Alloimmune Perspectives.

Transplantation·2026
Same author

Microvascular Inflammation in Kidney Transplantation.

Journal of the American Society of Nephrology : JASN·2026
Same author

Declining All-cause Allograft Survival in the Modern era: A 50-y Single-center Consecutive Cohort Study.

Transplantation·2026
Same author

The Evolution of Molecular Phenotypes Through Time in Kidney Transplant Recipients.

Transplantation direct·2026
Same author

Not all Mismatches Were Created Equal: Empirical Identification of Regions Within HLA-DQ Molecules That Harbor the Highest Immunogenicity.

Transplantation·2026

Related Experiment Video

Updated: Feb 16, 2026

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
11:28

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

41.6K

HLA Diagnostics: Evaluating DSA Strength by Titration.

Anat R Tambur1, Chris Wiebe2,3

  • 1Transplant Immunology Laboratory, Comprehensive Transplant Center, Northwestern University, Chicago, IL.

Transplantation
|December 22, 2017
PubMed
Summary

Measuring human leukocyte antigen (HLA) antibody strength offers more detailed insights than simple presence or absence. Titration studies provide valuable data across all transplant stages, enhancing clinical decision-making.

More Related Videos

Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
08:27

Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology

Published on: March 24, 2015

15.3K
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
10:37

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

3.4K

Related Experiment Videos

Last Updated: Feb 16, 2026

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
11:28

Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques

Published on: March 13, 2015

41.6K
Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology
08:27

Detection of Human Leukocyte Antigen Biomarkers in Breast Cancer Utilizing Label-free Biosensor Technology

Published on: March 24, 2015

15.3K
Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix
10:37

Analysis of HBV-Specific CD4 T-cell Responses and Identification of HLA-DR-Restricted CD4 T-Cell Epitopes Based on a Peptide Matrix

Published on: October 20, 2021

3.4K

Area of Science:

  • Immunology
  • Transplantation Science
  • Clinical Diagnostics

Background:

  • Donor-specific HLA antibodies are critical in transplant diagnostics and decision-making.
  • Simple detection of HLA antibodies lacks sufficient clinical granularity.
  • Quantifying HLA antibody strength is valuable throughout the transplant timeline.

Purpose of the Study:

  • To compare methods for evaluating HLA antibody strength.
  • To emphasize the clinical utility of HLA antibody titration studies.

Main Methods:

  • Comparison of IgG-mean fluorescence intensity (MFI) with serial dilution studies (titration).
  • Evaluation of C1q binding (C1q-MFI).
  • Assessment across pretransplant evaluation, donor evaluation, and posttransplant monitoring.

Main Results:

  • HLA antibody strength assessment provides greater clinical detail than binary presence/absence.
  • Titration studies offer significant added value at key transplant milestones.
  • C1q-MFI and titration provide complementary information on antibody activity.

Conclusions:

  • Quantifying HLA antibody strength is essential for comprehensive transplant management.
  • Titration studies are a valuable tool for assessing antibody potency at all transplant stages.
  • Integrating antibody strength assessment improves diagnostic accuracy and patient outcomes.