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Updated: Feb 16, 2026
09:51
Enhanced Genome Editing with Cas9 Ribonucleoprotein in Diverse Cells and Organisms
Published on: May 25, 2018
35.9K
Graphene oxide-mediated Cas9/sgRNA delivery for efficient genome editing
Huahua Yue1, Xiaoming Zhou, Meng Cheng
1MOE Key Laboratory of Laser Life Science & Institute of Laser Life Science, College of Biophotonics, South China Normal University, Guangzhou 510631, China. xingda@scnu.edu.cn zhouxm@scnu.edu.cn.
Nanoscale
|December 22, 2017
Summary
This study developed a novel graphene oxide (GO)-based nanocarrier for delivering CRISPR/Cas9 gene editing complexes. The system demonstrated efficient gene editing in human cells and enhanced stability for potential in vivo applications.
Area of Science:
- Biotechnology
- Nanomedicine
- Molecular Biology
Background:
- Direct cellular delivery of CRISPR/Cas9 is crucial for genome editing and gene regulation.
- Existing delivery methods face challenges in efficiency and stability.
Purpose of the Study:
- To develop and evaluate a novel nanocarrier for CRISPR/Cas9 delivery.
- To assess the efficiency and stability of the nanocarrier for gene editing applications.
Main Methods:
- Construction of a graphene oxide (GO)-polyethylene glycol (PEG)-polyethylenimine (PEI) nanocarrier.
- Delivery of Cas9/single-guide RNA (sgRNA) complexes into human AGS cells.
- Evaluation of endocytosis, endosomal escape, nuclear entry, and gene editing efficiency.
Main Results:
- The nanocarrier successfully delivered CRISPR/Cas9 complexes into human AGS cells.
- Achieved an efficient gene editing rate of approximately 39%.
- Demonstrated high stability by protecting sgRNA from enzymatic degradation.
Conclusions:
- The GO-PEG-PEI nanocarrier is a promising system for CRISPR/Cas9 delivery.
- This system offers enhanced stability for potential in vivo gene editing applications.
- Represents a novel approach for biomedical research and targeted gene engineering.
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