How has dental pulp stem cells isolation been conducted? A scoping review
Camila Perelló Ferrúa1, Eduarda Gervini Zampieri Centeno2, Laísa Camerini da Rosa1
1Universidade Católica de Pelotas - UCPel, Program in Health and Behavior, Pelotas, RS, Brazil.
Brazilian Oral Research
|December 22, 2017
Summary
This review highlights the variability in isolating dental pulp stem cells (DPSCs), hindering standardized protocols. Further research is needed to establish consistent methods for DPSC isolation and characterization.
Area of Science:
- Regenerative Medicine
- Stem Cell Biology
- Biotechnology
Background:
- Dental pulp stem cells (DPSCs) are a promising source for regenerative therapies.
- Variability in isolation methods complicates the standardization of DPSC research.
- Understanding isolation profiles is crucial for clinical applications.
Purpose of the Study:
- To conduct a scoping review of literature on DPSC isolation.
- To identify common isolation profiles and methodologies for DPSCs.
- To analyze potential risk factors affecting DPSC behavior.
Main Methods:
- Comprehensive literature search using MeSH terms in PubMed, Scopus, Scielo, and ISI Web of Knowledge.
- Inclusion and exclusion criteria applied to select relevant studies.
- Scoping review methodology to synthesize findings from 222 articles.
Main Results:
- Significant variability exists in current DPSC isolation techniques.
- No standardized protocol for DPSC isolation has been widely adopted.
- The past 15 years have seen extensive research on DPSCs, but this is the first systematic review of isolation.
Conclusions:
- The heterogeneity in DPSC isolation methods impedes the development of reproducible protocols.
- Standardization is essential for advancing the therapeutic potential of DPSCs.
- This review consolidates isolation methodologies, providing a foundation for future standardization efforts.
