Related Experiment Video
Updated: Feb 16, 2026
14:14
Adaptation of Semiautomated Circulating Tumor Cell CTC Assays for Clinical and Preclinical Research Applications
Published on: February 28, 2014
16.3K
Cell Cycle Model System for Advancing Cancer Biomarker Research
Iulia M Lazar1,2, Ina Hoeschele3, Juliana de Morais4
1Department of Biological Sciences, Virginia Tech. 1981 Kraft Drive, Blacksburg, VA, 24061, USA. malazar@vt.edu.
Scientific Reports
|December 23, 2017
Summary
This study shows how combining public cancer data with mass spectrometry can identify functionally related protein markers. These findings support developing new molecular assays for early cancer detection and precision medicine.
Area of Science:
- Biochemistry
- Molecular Biology
- Bioinformatics
Background:
- Understanding cancer complexity necessitates advanced molecular markers for early detection and personalized medicine.
- Public data mining and mass spectrometry offer powerful tools for biomarker discovery.
Purpose of the Study:
- To determine if protein markers from public data mining can be biologically contextualized.
- To assess mass spectrometry's utility in screening for co-expressed, functionally related biomarkers for cancer.
Main Methods:
- Utilized cell cycle arrest/release experiments in breast cancer and non-tumorigenic cell lines.
- Queried public domain information using bioinformatics tools to generate a list of cancer-associated proteins.
- Performed mass spectrometric analysis on cell extracts and analyzed proteomic data for differential expression and interactions.
Main Results:
- Identified 352 proteins from cell extracts matching an initial list of 1038 cancer-associated proteins.
- Revealed functionally related protein clusters relevant to cancer through differential expression, enrichment, and protein-protein interaction analysis.
- Demonstrated the biological relevance of public data for guiding research.
Conclusions:
- Publicly available data can effectively inform biological research and biomarker discovery.
- Mass spectrometry is a viable method for screening co-expressed, functionally related protein biomarkers.
- This approach supports the development of molecular assays for cancer characterization and clinical applications.
Related Concept Videos
01:28The Cell Cycle Control System
5.8K
The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and...
5.8K
02:12What is Cancer?
14.5K
Cells and tissues must meticulously coordinate their activities for the normal functioning of the human body. Therefore, they exhibit socially responsible behavior - resting, growing, dividing, differentiating, or dying - for the organism’s benefit. Cancer arises when cells divide uncontrollably and invade other tissues or organs.
Although people have known about cancer for centuries, it was only in 1761 that Giovanni Morgagni of Padua performed a detailed autopsy of...
Although people have known about cancer for centuries, it was only in 1761 that Giovanni Morgagni of Padua performed a detailed autopsy of...
14.5K