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CD60b: Enriching Neural Stem/Progenitor Cells from Rat Development into Adulthood.
Fernanda Gubert1, Camila Zaverucha-do-Valle1, Michelle Furtado1
1Instituto de Biofísica Carlos Chagas Filho, Universidade Federal do Rio de Janeiro, 21941-902 Rio de Janeiro, RJ, Brazil.
Stem Cells International
|December 23, 2017
Summary
CD60b antigens, particularly ganglioside 9-O-acetyl GD3, are present in neural stem/progenitor cells. This discovery aids in identifying and isolating these cells for potential regenerative therapies in the central nervous system.
Area of Science:
- Neuroscience
- Developmental Biology
- Cell Biology
Background:
- CD60b antigens show high expression during nervous system development in rats.
- In adult rats, CD60b expression is confined to specific regions like the subventricular zone (SVZ), a known neurogenic niche.
Purpose of the Study:
- To investigate the association between CD60b expression and neural stem/progenitor cells in the rat SVZ from development through adulthood.
- To determine if CD60b can be utilized for the isolation and enrichment of neural stem/progenitor cells.
Main Methods:
- In vitro and in vivo analyses of CD60b expression across different developmental stages.
- Purification and enrichment of neurosphere-forming cells using CD60b as a marker.
Main Results:
- CD60b antigens were identified in neural stem/progenitor cells in both developing and adult rat brains.
- CD60b facilitated the purification and enrichment of neurosphere-forming cells.
- CD60b, primarily ganglioside 9-O-acetyl GD3, is present in less mature cells and can identify/isolate neural stem/progenitor cells.
Conclusions:
- CD60b is a valuable marker for identifying and isolating neural stem/progenitor cells in the developing and adult mammalian brain.
- Understanding CD60b's role in neurogenesis can advance knowledge of central nervous system physiology.
- This research may lead to new therapeutic strategies for tissue regeneration after neurological injury or disease.
