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Macromolecular Coating Enables Tunable Selectivity in a Porous PDMS Matrix
Benjamin Winkeljann1, Benjamin T Käsdorf1, Job Boekhoven2
1Department of Mechanical Engineering and Munich School of Bioengineering, Technical University of Munich, Boltzmannstraße 11, 85748, Garching, Germany.
Macromolecular Bioscience
|December 23, 2017
Summary
This study introduces a tunable, PDMS-based capillary filter system for life sciences. The novel design enables selective filtering and enzyme immobilization, enhancing lab efficiency.
Area of Science:
- Biomaterials Science
- Chemical Engineering
- Life Sciences
Background:
- Selective filtration is crucial in life sciences but current systems lack tunability.
- High surface-to-volume ratio and specific chemical groups are key for efficient filtering.
Purpose of the Study:
- To develop an artificial polydimethylsiloxane (PDMS)-based capillary system with tunable selectivity.
- To demonstrate its utility in selective filtration and enzyme immobilization.
Main Methods:
- Fabrication of a porous PDMS matrix using embedded sugar fibers for a high surface-to-volume ratio.
- Functionalization of the capillary inner surface with macromolecules for selective binding.
- Immobilization of enzymes within the capillary system.
Main Results:
- Demonstrated tunable selective sieving based on steric hindrance, electrostatic, and specific binding interactions.
- Showcased successful enzyme immobilization for multiple reaction cycles without product separation.
- Validated the system for lab-scale filtration and enzyme applications.
Conclusions:
- The developed PDMS capillary system offers highly tunable filtration properties.
- It provides a versatile platform for selective separation, enzyme immobilization, and binding interaction studies.
- This technology has potential applications in both laboratory and clinical settings.
