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SOX7 Suppresses Wnt Signaling by Disrupting β-Catenin/BCL9 Interaction

Rong Fan1, HaiYan He2, Wang Yao1

  • 11 Department of Cardiology, Yueyang Hospital Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine , Shanghai, China .

DNA and Cell Biology
|December 23, 2017
PubMed

Insights

Sex-determining region Y-box 7 (SOX7) inhibits Wnt signaling, a pathway crucial for tumor development. SOX7 disrupts the interaction between β-catenin and BCL9, acting as a tumor suppressor and potential anticancer target.

Area of Science:

  • Molecular Biology
  • Cancer Research
  • Cell Signaling

Background:

  • Wnt signaling pathway is implicated in angiogenesis and tumor progression.
  • β-catenin is a key mediator of oncogenic Wnt signaling.
  • The precise mechanism by which SOX7 inhibits Wnt signaling in tumors is not fully understood.

Purpose of the Study:

  • To elucidate the mechanism by which Sex-determining region Y-box 7 (SOX7) inhibits oncogenic Wnt signaling.
  • To investigate the interaction between SOX7, β-catenin, and BCL9.
  • To evaluate SOX7 as a potential therapeutic target for cancer.

Main Methods:

  • Co-immunoprecipitation (co-IP) assays to assess protein interactions.
  • Super Topflash reporter assays to measure Wnt/β-catenin transcriptional activity.
  • Analysis of SOX7's effect on the β-catenin/BCL9 complex.

Main Results:

  • SOX7 directly binds to β-catenin.
  • SOX7 inhibits β-catenin/T cell factor (TCF)-mediated transcription.
  • SOX7 disrupts the interaction between β-catenin and BCL9 by competing for β-catenin binding.
  • SOX7 functions as a suppressor of oncogenic Wnt signaling.

Conclusions:

  • SOX7 inhibits oncogenic Wnt signaling by preventing the β-catenin/BCL9 interaction.
  • SOX7 acts as a tumor suppressor by targeting the Wnt pathway.
  • SOX7 represents a promising therapeutic target for anticancer strategies.
Keywords:
BCL9SOX7Wnt signalingcancerβ-catenin

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