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Area of Science:

  • Immunology
  • Microbiology
  • Bacterial Pathogenesis

Background:

  • Staphylococcus aureus evades human neutrophil killing, contributing to disease.
  • The SaeR/S two-component system in S. aureus influences host immune responses.

Purpose of the Study:

  • To investigate the role of S. aureus SaeR/S system in modulating monocyte TNF-α production.
  • To determine the impact of SaeR/S on human neutrophil priming and bactericidal activity.

Main Methods:

  • Flow cytometry and ELISA were used to measure TNF-α production by peripheral blood mononuclear cells (PBMCs).
  • Neutrophil priming and bactericidal assays utilized conditioned media from PBMC-bacteria co-cultures.

Main Results:

  • S. aureus USA300 significantly reduced monocyte TNF-α production compared to a saeR/S deletion mutant (USA300∆saeR/S).
  • Neutrophils primed with media from USA300∆saeR/S-stimulated PBMCs showed enhanced bactericidal activity against USA300.
  • Increased neutrophil killing correlated with elevated reactive oxygen species and TNF-α levels.

Conclusions:

  • S. aureus employs the SaeR/S system as an immune evasion strategy.
  • This system suppresses TNF-α, impairing neutrophil priming and bacterial killing.