Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Allergic Reactions02:06

Allergic Reactions

32.5K
Overview
32.5K
Allergic Reactions: Anaphylaxis01:30

Allergic Reactions: Anaphylaxis

18
Anaphylaxis is a severe, life-threatening hypersensitivity reaction mediated by Immunoglobulin E (IgE) antibodies. When IgE binds to allergens, it triggers the release of mediators– histamine, leukotrienes, and prostaglandins from mast cells and basophils. These mediators cause vasodilation, edema, and inflammation, leading to various symptoms.The primary allergens causing anaphylaxis include food items (e.g., peanuts, shellfish), drugs (e.g., penicillin, asparaginase, corticotropin,...
18
Drug Toxicity: Allergic Reactions01:30

Drug Toxicity: Allergic Reactions

16
Drug-related allergies are immune-mediated responses triggered by the administration of pharmacological agents. These hypersensitivity reactions are classified based on the immune mechanisms involved. The four primary types—Type I, II, III, and IV—are mediated by different immunological pathways and exhibit distinct clinical manifestations.Type I Hypersensitivity/ IgE-Mediated Reactions: Immunoglobulin E (IgE) immediately mediates Type I hypersensitivity reactions. Upon initial...
16
Cross-reactivity00:42

Cross-reactivity

33.2K
Overview
33.2K
Allergic Drug Reactions01:27

Allergic Drug Reactions

1.5K
Allergic reactions related to drugs are hypersensitivity responses driven by the immune system and bear no connection to the drug's therapeutic action. While drugs in isolation do not trigger an immune response, they can interact with endogenous proteins to form antigens. These antigens stimulate lymphocytes to produce antibodies. IgE-type antibodies attach themselves to mast cells. Upon subsequent exposure to the same stimulus, the antigen-antibody interaction is initiated, unleashing...
1.5K
Hypersensitivities01:30

Hypersensitivities

7.8K
Hypersensitivity, also known as a hypersensitivity reaction or allergic reaction, is a condition where the body's immune system reacts abnormally to a foreign substance. Such substances, that cause hypersensitivity are referred to as an allergen, could be something typically harmless to most people, like pollen or certain foods.
Types of Hypersensitivities
Hypersensitivity reactions are categorized into four types: Type 1, Type 2, Type 3, and Type 4. Each type has a distinct mechanism...
7.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Benralizumab and the integrated management of co-morbid severe eosinophilic asthma with chronic rhinosinusitis with nasal polyps.

Clinical and translational allergy·2025
Same author

Peanut-specific IgG subclasses as biomarkers of peanut allergy in LEAP study participants.

The World Allergy Organization journal·2024
Same author

Outcomes of intravenous immunoglobulin treatment of immunocompromised patients with viral respiratory infections.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology·2024
Same author

A case report and systematic literature review: insulin-induced type III hypersensitivity reaction.

Frontiers in allergy·2024
Same author

Tough Nut to Crack: Transplant-acquired Food Allergy in an Adult Liver Recipient.

Transplantation direct·2023
Same author

Randomized double-blind pilot study of universal, species abundant, multiallergen subcutaneous immunotherapy for moderate-severe allergic rhinitis.

Annals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology·2023

Related Experiment Video

Updated: Feb 16, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

17.0K

Reliability of allergy skin testing.

Maria Shtessel1, Jody Tversky1

  • 1Department of Medicine, Division of Allergy and Clinical Immunology, Johns Hopkins University, Baltimore, Maryland.

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
|December 24, 2017
PubMed
Summary

Higher histamine concentrations in skin tests may mask antihistamine effects, leading to inaccurate allergy diagnoses. Using a lower histamine control (1 mg/mL) can improve the detection of false-negative allergen responses in patients taking antihistamines.

More Related Videos

Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

9.6K
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

4.0K

Related Experiment Videos

Last Updated: Feb 16, 2026

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE
07:10

Application of Biochip Microfluidic Technology to Detect Serum Allergen-specific Immunoglobulin E sIgE

Published on: April 21, 2019

17.0K
Basophil Activation Test for Allergy Diagnosis
07:22

Basophil Activation Test for Allergy Diagnosis

Published on: May 31, 2021

9.6K
Humanized Mediator Release Assay as a Read-Out for Allergen Potency
10:22

Humanized Mediator Release Assay as a Read-Out for Allergen Potency

Published on: June 29, 2021

4.0K

Area of Science:

  • Allergy and Immunology
  • Dermatology
  • Pharmacology

Background:

  • Percutaneous allergen skin testing is a standard diagnostic method for atopic diseases.
  • Skin test reliability is influenced by allergen extracts, testing methods, and antihistamine use.

Purpose of the Study:

  • To investigate the differential impact of cetirizine on histamine controls and common allergens in percutaneous skin testing.
  • To assess the effect of cetirizine on wheal and flare responses at different histamine concentrations.

Main Methods:

  • Twelve participants underwent skin testing with varying histamine concentrations (1 and 6 mg/mL) and common aeroallergens.
  • Wheal and flare responses were measured after 4 days of cetirizine administration.
  • Measurements were taken in a masked fashion by a single operator.

Main Results:

  • Cetirizine suppressed wheal and flare responses, with maximum effect observed on day 5.
  • Histamine suppression was more pronounced with 1 mg/mL (62%) compared to 6 mg/mL (33%).
  • Higher histamine concentrations (6 mg/mL) led to more false-negative allergen responses, potentially indicating antihistamine presence.

Conclusions:

  • A 6 mg/mL histamine control may lead to false-negative allergen responses by failing to detect antihistamines.
  • Lower histamine concentrations (1 mg/mL) may be more effective in identifying antihistamine interference during skin testing.