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Surface coating affects uptake of silver nanoparticles in neural stem cells
Igor M Pongrac1, Lada Brkić Ahmed1, Hrvoje Mlinarić1
1University of Zagreb School of Medicine, Croatian Institute for Brain Research, Šalata 12, 10000, Zagreb, Croatia.
Summary
Surface coatings on silver nanoparticles (AgNPs) significantly alter their toxicity and cellular uptake in neural stem cells. Macropinocytosis is the primary uptake pathway for these AgNPs.
Area of Science:
- Nanotechnology
- Materials Science
- Toxicology
Background:
- Nanoparticle development requires safety assessments.
- Surface properties of metallic nanoparticles influence biological interactions.
- Understanding nanoparticle behavior in neural cells is crucial.
Purpose of the Study:
- To investigate the impact of various surface coatings on silver nanoparticle (AgNP) cytotoxicity.
- To analyze the cellular uptake patterns of different AgNPs in murine neural stem cells (mNSCs).
- To elucidate the role of surface chemistry in AgNP-cell interactions.
Main Methods:
- Synthesis of AgNPs stabilized with AOT, CTAB, PVP, PLL, and BSA.
- Assessment of cytotoxicity in mNSCs exposed to different AgNPs.
- Evaluation of cellular uptake mechanisms, including macropinocytosis.
Main Results:
- Different surface coatings on AgNPs resulted in varied cytotoxicity effects.
- AgNP internalization patterns differed based on surface stabilization.
- Macropinocytosis was identified as the predominant cellular uptake mechanism for all tested AgNPs in mNSCs.
Conclusions:
- Surface coatings are critical determinants of AgNP safety and cellular interactions.
- The findings provide essential data for the safety evaluation of novel nanomaterials.
- This research aids in designing safer nanoparticles for potential therapeutic or research applications.
