Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Chronic Bowel Disorders: Introduction01:17

Chronic Bowel Disorders: Introduction

817
Chronic bowel diseases are a group of long-term conditions affecting the digestive tract, characterized by inflammation and damage to the gut lining. These conditions primarily include irritable bowel syndrome and inflammatory bowel disease.
Irritable Bowel Syndrome (IBS) is a common disorder affecting the gastrointestinal tract. The distinctive feature is recurrent abdominal pain associated with altered bowel movements, manifesting as constipation, diarrhea, or fluctuating between both. The...
817
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.7K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.7K
Cytoskeletal Linker Proteins - Plakins01:09

Cytoskeletal Linker Proteins - Plakins

2.9K
Plakins are large proteins with binding domains for microtubules, microfilaments, intermediate filaments, and membrane-associated protein complexes at cell junctions. Plakin functions are evolutionarily conserved and are primarily involved in organizing the different components of the cytoskeleton by crosslinking them to each other and connecting them to the cell-matrix and cell adhesion complexes. They are also known to interact with signal transducers, serve as scaffolds for signaling...
2.9K
Phosphoinositides and PIPs01:42

Phosphoinositides and PIPs

10.3K
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
10.3K
Chronic Pancreatitis I: Introduction01:24

Chronic Pancreatitis I: Introduction

754
The pancreas, an elongated and flat gland situated behind the stomach, serves a vital function in digesting food and managing blood sugar levels.
Pancreatitis is the inflammation of the pancreas, which occurs when the immune system becomes active and causes swelling, pain, and disruptions in organ function. Pancreatitis can manifest as either an acute or chronic condition.
Acute pancreatitis arises suddenly and lasts for a brief duration, while chronic pancreatitis is a long-term affliction...
754
Pleiotropy01:33

Pleiotropy

43.4K
Pleiotropy is the phenomenon in which a single gene impacts multiple, seemingly unrelated phenotypic traits. For example, defects in the SOX10 gene cause Waardenburg Syndrome Type 4, or WS4, which can cause defects in pigmentation, hearing impairments, and an absence of intestinal contractions necessary for elimination. This diversity of phenotypes results from the expression pattern of SOX10 in early embryonic and fetal development. SOX10 is found in neural crest cells that form melanocytes,...
43.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A comparative study of machine and deep learning models for time-series-based bearing fault diagnosis of induction motors.

Scientific reports·2026
Same author

Perinatal LC-ω-3-PUFA supplementation offsets prenatal THC-induced cognitive deficit via sex-specific modulation of hippocampal neuroplasticity.

European journal of pharmacology·2026
Same author

Fibrillar adhesion dynamics govern the timescales of nuclear mechano-response via the vimentin cytoskeleton.

Nature materials·2026
Same author

Multilevel impact of cocaine on honeybees: From neurochemistry and mitochondrial dysfunction to superorganism.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie·2026
Same author

Multidimensional analysis of social withdrawal in the sub-chronic phencyclidine rat model for schizophrenia.

Psychopharmacology·2026
Same author

Metabolic profile of 25E-NBOH in human liver microsomes, rat urine, and fungus Cunninghamella elegans.

Journal of pharmaceutical and biomedical analysis·2026

Related Experiment Video

Updated: Feb 16, 2026

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology
12:36

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Published on: March 14, 2020

17.8K

Plectin controls biliary tree architecture and stability in cholestasis.

Marketa Jirouskova1, Katerina Nepomucka2, Gizem Oyman-Eyrilmez1

  • 1Laboratory of Integrative Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, Prague, Czech Republic.

Journal of Hepatology
|December 24, 2017
PubMed
Summary

Plectin protein is crucial for liver health, maintaining keratin structure and biliary epithelial stability. Its absence worsens liver damage during cholestasis, highlighting its protective role.

Keywords:
Bile duct ligationCell-cell junctionsCytolinkerDDC dietKeratin filaments

More Related Videos

Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis
04:38

Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis

Published on: March 28, 2018

16.6K
Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
10:12

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Published on: December 20, 2019

12.5K

Related Experiment Videos

Last Updated: Feb 16, 2026

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology
12:36

Murine Precision-Cut Liver Slices as an Ex Vivo Model of Liver Biology

Published on: March 14, 2020

17.8K
Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis
04:38

Partial Bile Duct Ligation in the Mouse: A Controlled Model of Localized Obstructive Cholestasis

Published on: March 28, 2018

16.6K
Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro
10:12

Isolation and 3D Collagen Sandwich Culture of Primary Mouse Hepatocytes to Study the Role of Cytoskeleton in Bile Canalicular Formation In Vitro

Published on: December 20, 2019

12.5K

Area of Science:

  • Cell Biology
  • Hepatology
  • Biochemistry

Background:

  • Plectin is a versatile cytolinker protein regulating intermediate filament organization and cellular stress responses.
  • Its specific role in liver physiology, particularly during cholestasis, remained largely uncharacterized.

Purpose of the Study:

  • To investigate the function of plectin in the liver under normal conditions and during experimental cholestasis.
  • To elucidate the impact of plectin deficiency on keratin filament organization and biliary epithelial integrity.

Main Methods:

  • Generation of liver-specific plectin knockout (PleΔalb) mice.
  • Utilized bile duct ligation (BDL) and DDC feeding models for cholestatic liver injury.
  • In vitro studies using primary hepatocytes and cholangiocyte cell lines.

Main Results:

  • Plectin deficiency caused aberrant keratin networks, biliary malformations, and aggravated cholestatic liver injury.
  • Plectin-deficient bile ducts showed dilation, rupture, and increased bile infarcts.
  • Compromised tight junctions and altered cell adhesion molecule expression were observed in plectin-deficient cells.

Conclusions:

  • Plectin is essential for maintaining keratin cytoarchitecture and biliary epithelial stability in the liver.
  • Plectin plays a critical protective role against cholestasis-induced liver stress.