A New Multi-Mode Perfusion System for Ex Vivo Heart Perfusion Study

Area of Science:

• Cardiovascular Surgery
• Biomedical Engineering
• Organ Transplantation

Background:

• Ex vivo heart perfusion aids donor heart resuscitation and assessment for transplantation.
• Limited development of versatile ex vivo perfusion systems exists.
• A single platform for multiple perfusion strategies is needed.

Purpose of the Study:

• To design, develop, and test a novel, modular ex vivo perfusion system.
• To enable multiple perfusion strategies on a single platform.
• To evaluate the system's efficacy in preserving donor hearts.

Main Methods:

• Developed a modular ex vivo perfusion system with Langendorff, Pump-Supported Working-Mode, and Passive Afterload Working-Mode.
• Conducted six experiments using porcine hearts.
• Measured hemodynamic parameters (aortic pressure) and metabolic markers (pH, lactate).
• Assessed left ventricular contractility using pressure-volume loops and coronary vascular resistance (CVR).

Main Results:

• The system operated effectively in three distinct perfusion modes.
• Physiological hemodynamic parameters were achievable by adjusting settings.
• Hemodynamic and metabolic conditions remained stable for 4 hours.
• Left ventricular contractility was assessed in Working Modes.

Conclusions:

• The novel modular ex vivo perfusion system is a viable platform.
• It supports multiple perfusion strategies for donor heart preservation and assessment.
• Further development of novel preservation and assessment strategies is warranted.