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Complete culture medium is better than low folate medium for detecting increased chromosome aberrations in smokers in
J A Reidy1, H G Zhou, A T Chen
1Division of Environmental Health Laboratory Sciences, Centers for Disease Control, Atlanta, GA 30333.
Mutation Research
|April 1, 1989
Abstract:
We compared chromosome breakage in parallel, 48-h lymphocyte cultures established from smokers and nonsmokers using minimal essential medium (MEM) and MEM without folate (MEM-FA). There was a statistically significant, higher frequency of aberrations for smokers than for nonsmokers in cells cultured in MEM, but not in those cultured in MEM-FA. Thus, these data support the recommendation of the World Health Organization (1985) that population monitoring studies for exposure assessment should not use a low folate medium.