Related Experiment Video
Updated: Feb 16, 2026

Hot Biological Catalysis: Isothermal Titration Calorimetry to Characterize Enzymatic Reactions
Published on: April 4, 2014
Enzymatic characterization and functional analysis of EcChi3C from ridgetail white prawn Exopalaemon carinicauda
Yuying Sun1, Jiquan Zhang2, Fengge Song3
1Jiangsu Key Laboratory of Marine Pharmaceutical Compound Screening, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang, 222005, China; College of Life Sciences, Hebei University, Baoding, Hebei, 071002, China; College of Marine Life and Fisheries, Huaihai Institute of Technology, 59 Cangwu Road, Lianyungang, 222005, China; Co-Innovation Center of Jiangsu Marine Bio-industry Technology, Huaihai Institute of Technology, Lianyungang, 222005, China.
Abstract:
Chitinase belongs to the glycosyl hydrolases family 18 and plays key role in the development and pathogen resistance of crustaceans. In this study, the enzymatic characterization of chitinase 3C (EcChi3C) of Exopalaemon carinicauda was analyzed. In addition, we analyzed the expression profiles of EcChi3C at different tissues and different molting stages. In the all tested tissues, it was predominantly expressed in hepatopancreas, and then stomach, but poor in other tissues. In all tested molting periods, it was mainly expressed in intermolt and molting stages, but poor in other stages. The results of molting, mortality and the uropod ultrastructure of prawns after being injected with EcChi3C dsRNA were in accordance with those of the control group. In addition, there is no difference for endopodite morphology between the survival and dead individuals in experimental group. After being challenged with bacteria, the expression of EcChi3C was up-regulated significantly at 12 h and followed with a comeback at 96 h. These results suggest that EcChi3C is an important immune related gene but not a necessary gene in the molting process of E. carinicauda.
Related Concept Videos
Amplifying Signals via Enzymatic Cascade
Performing a Simple Data Analysis using MS-Excel Function
SUM: This function calculates the total sum of a range of values. It's the foundation for aggregating data, essential for determining overall trends and totals in datasets.
AVERAGE: It computes the mean value of a given set of numbers, providing a quick insight into the central...
Functional Groups
Functional Groups
Functionalism
James envisioned psychology's...
Mechanical Protein Functions

