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Updated: Feb 16, 2026

Optical Detection of E. coli Bacteria by Mesoporous Silicon Biosensors
Published on: November 20, 2013
Rose petal and P123 dual-templated macro-mesoporous TiO2 for a hydrogen peroxide biosensor
Xu Wu1, Huanhuan Zhang2, Kejing Huang3
1College of Physics and Electronic Engineering, Xinyang Normal University, Xinyang 464000, PR China.
Abstract:
In this work, highly ordered macro-mesoporous TiO2 has been successfully synthesized using fresh rose petals and P123 (EO20PO70EO20) as dual templates through a simple soaking and calcining process. Characterization of the as-prepared TiO2 indicated that the mesoporous structure of the TiO2 was highly ordered, with a pore diameter of approximately 3nm. After electrodeposition of Pt nanoparticles onto the TiO2 as an electron transfer enhancer and the immobilization of horseradish peroxidase (HRP) onto the TiO2-modified electrode, a biosensor for detecting hydrogen peroxide (H2O2) was realized. This biosensor showed a wide linear detection range from 5μM to 8mM and a low detection limit of 1.65μM with good stability and high selectivity, suggesting that the sensor is well-suited for the detection of H2O2.
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