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Bacterial Expression and Purification of Human Matrix Metalloproteinase-3 using Affinity Chromatography
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Interferon regulatory factor 5 (IRF5) regulates the expression of matrix metalloproteinase-3 (MMP-3) in human

Lin Guo1, Ruihu Hao2, Fengde Tian2

  • 1Department of Orthopedics, The Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, China.

International Immunopharmacology
|December 24, 2017
PubMed

Insights

Interferon regulatory factor 5 (IRF5) promotes matrix metalloproteinase-3 (MMP-3) expression in osteoarthritis. IRF5 may be a new therapeutic target for osteoarthritis and rheumatoid arthritis.

Area of Science:

  • Molecular biology
  • Immunology
  • Rheumatology

Background:

  • Matrix metalloproteinase-3 (MMP-3) is key in osteoarthritis (OA) cartilage destruction.
  • Regulation of MMP-3 gene expression is complex.
  • The role of Interferon regulatory factor 5 (IRF5) in chondrocytes and OA is largely unknown.

Purpose of the Study:

  • To investigate the role of IRF5 in OA pathogenesis.
  • To determine if IRF5 regulates MMP-3 expression in chondrocytes.

Main Methods:

  • IRF5 expression analysis in human chondrocytes and cell lines.
  • Investigated IRF5 regulation by TNF-α.
  • Assessed IRF5's effect on MMP-3 expression via overexpression and knockdown.
  • Analyzed IRF5 binding to the MMP-3 promoter.
  • Examined the involvement of NF-κB.

Main Results:

  • IRF5 is expressed in human chondrocytes and upregulated by TNF-α.
  • IRF5 levels are higher in OA chondrocytes compared to normal.
  • IRF5 directly enhances MMP-3 transcription by binding to its promoter.
  • IRF5 mediates TNF-α-induced MMP-3 expression, involving NF-κB.

Conclusions:

  • IRF5 plays a significant role in MMP-3 expression in chondrocytes.
  • IRF5 is a potential therapeutic target for osteoarthritis (OA) and rheumatoid arthritis (RA).
Keywords:
ChondrocytesInterferon regulatory factor 5 (IRF5)Matrix metalloproteinase-3Osteoarthritis

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