Related Experiment Video
Updated: Feb 16, 2026

GC-based Detection of Aldononitrile Acetate Derivatized Glucosamine and Muramic Acid for Microbial Residue Determination in Soil
Published on: May 19, 2012
[Determination of Methcathinone in Urine by GC-MS]
1Department of Criminal Science and Technology, Hunan Police Academy, Changsha 410138, China.
Objectives:
To establish a method for the analysis of methcathinone in urine by gas chromatography-mass spectrometry (GC-MS).
Methods:
Proadifen hydrochloride (internal standard) and buffer solution (pH=9) were added into the urine samples, and methcathinone was extracted by ethyl acetate. The extract was volatilized in 50 ℃ nitrogen gas flow and the remnant was dissolved by methanol and analysed by GC-MS.
Results:
The methcathinone in urine showed a good linear relationship in the mass concentration range of 0.02-2.00 μg/mL. The linear equation was y=0.301 9 x+0.018 9 (r=0.999 2), and the detection limit was 0.01 μg/mL. The recoveries of methcathinone in urine was 96.4%-99.2%, with the intra-day precision of 5.8%-7.6% and the inter-day precision of 6.0%-8.1%.
Conclusions:
The method is convenient and sensitive, which can be applied to the forensic identification of methcathinone in urine.
Related Concept Videos
Filtration and Urine Formation
Urine Studies II: Urine Culture and Sensitivity Test
Urine Studies I: Urinalysis
Physiology of Urine Formation
Glomerular Filtration
The first stage in urine formation is glomerular filtration. Each kidney contains approximately 1 million nephrons, the functional units of filtration, with a...
Formation of Concentrated Urine
Formation of Dilute Urine
Filtrate Osmolarity in the PCT
Initially, as the filtrate passes through the proximal convoluted tubule (PCT), its...

