Related Experiment Video

Updated: Feb 16, 2026

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice
07:36

Induction of Atherosclerotic Plaques Through Activation of Mineralocorticoid Receptors in Apolipoprotein E-deficient Mice

Published on: September 26, 2018

10.6K

Low-Density Lipoprotein Receptor Deficiency Attenuates Neuroinflammation through the Induction of Apolipoprotein E

Jo Mailleux1, Silke Timmermans1, Katherine Nelissen1

  • 1Biomedical Research Institute, Hasselt University, Diepenbeek, Belgium.

Frontiers in Immunology
|December 26, 2017
PubMed
Abstract

Insights

Low-density lipoprotein receptor (LDLr) deficiency attenuated experimental autoimmune encephalomyelitis (EAE) in female mice by increasing apolipoprotein E (apoE). This highlights LDLr

Area of Science:

  • Neuroimmunology
  • Lipid Metabolism
  • Autoimmune Diseases

Background:

  • Neuroinflammation is a key component of multiple sclerosis and other neurological disorders.
  • The low-density lipoprotein receptor (LDLr) plays a role in cholesterol homeostasis.
  • The involvement of LDLr in neuroinflammatory processes remains incompletely understood.

Purpose of the Study:

  • To investigate the role of LDLr in neuroinflammation using a mouse model of experimental autoimmune encephalomyelitis (EAE).
  • To determine the impact of LDLr deficiency on EAE disease severity and underlying inflammatory mechanisms.

Main Methods:

  • Induction of MOG35-55 EAE in LDLr knockout (ldlr-/-) mice (male and female).
  • Clinical and histopathological assessment of EAE.
  • Quantitative PCR (qPCR) for inflammatory mediators and apolipoprotein E (apoE).
  • Western blot and ELISA for apoE and TNFα protein levels.

Main Results:

  • LDLr deficiency attenuated EAE severity in female mice, but not in males.
  • Female ldlr-/- mice exhibited reduced central nervous system proinflammatory cytokine production.
  • Macrophages from female ldlr-/- mice showed decreased proinflammatory mediators, impaired myelin phagocytosis, and enhanced apoE secretion.
  • ApoE/LDLr double knockout abrogated the protective effect of LDLr depletion in EAE.

Conclusions:

  • LDLr deficiency reduces EAE severity in female mice, linked to increased apoE levels.
  • The observed sexual dimorphism in EAE response warrants further investigation.
  • LDLr and apoE are implicated in modulating neuroinflammatory processes.
Keywords:
apolipoprotein Eexperimental autoimmune encephalomyelitislow-density lipoprotein receptormultiple sclerosisneuroinflammation

More Related Videos

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation06:47

Isolation and Analysis of Plasma Lipoproteins by Ultracentrifugation

Published on: January 28, 2021

12.5K
Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification10:39

Isolation of High-density Lipoproteins for Non-coding Small RNA Quantification

Published on: November 28, 2016

11.8K

Related Concept Videos

Receptor-mediated Endocytosis01:38

Receptor-mediated Endocytosis

Overview
111.6K
Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.6K
Density00:56

Density

Density is an important characteristic of substances, crucial in determining whether an object sinks or floats in a fluid. Its SI unit is kg/m3, and its cgs unit is g/cm3. The density of an object helps in identifying its composition, and also reveals information about the phase of the matter and its substructure. The densities of liquids and solids are roughly comparable, consistent with the fact that their atoms are in close contact. However, gases have much lower densities than liquids and...
20.0K
Internal Receptors01:31

Internal Receptors

Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.8K
Current Density01:21

Current Density

The total amount of current flowing through one unit value of a cross-sectional area is referred to as current density. If the current flow is uniform, the amount of current flowing through a conductor is the same at all points along the conductor, even if the conductor area varies. The current density consists of the local magnitude and direction of the charge flow, which varies from point to point. Current density is measured in amperes per meter square, and direction is defined as the net...
5.2K
Induction01:16

Induction

An emf is induced when the magnetic field in a coil is changed by pushing a bar magnet into or out of the coil. emfs of opposite signs are produced by motion in opposite directions, and the directions of emfs are also reversed by reversing poles. The same results are produced if the coil is moved rather than the magnet—it is the relative motion that is important. The faster the motion, the greater the emf. Additionally, there is no emf when the magnet is stationary relative to the coil.
A...
5.8K