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In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice
Published on: February 20, 2020
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Drosophila Model of Leishmania amazonensis Infection
1Division of Infectious Diseases, Department of Medicine, University of Massachusetts Medical School, Worcester, MA, USA.
Bio-Protocol
|December 26, 2017
Abstract:
This protocol describes how to generate and harvest antibody-free L. amazonensis amastigotes, and how to infect adult Drosophila melanogaster with these parasites. This model recapitulates key aspects of the interactions between Leishmania amastigotes and animal phagocytes.

