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Anaerobic Protein Purification and Kinetic Analysis via Oxygen Electrode for Studying DesB Dioxygenase Activity and Inhibition
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Singlet oxygen luminescence kinetics under PDI relevant conditions of pathogenic dermatophytes and molds

Tobias Bornhütter1, Nedaa Shamali1, Irena Saltsman2

  • 1Department of Physics, Humboldt-Universität zu Berlin, Newtonstraße 15, 12489 Berlin, Germany.

Journal of Photochemistry and Photobiology. B, Biology
|December 26, 2017
PubMed

Insights

Photodynamic therapy offers a promising alternative for treating onychomycosis. This study explored photosensitizer behavior with fungi, detecting singlet oxygen luminescence for up to seven days, paving the way for effective treatments.

Area of Science:

  • Photodynamic therapy
  • Medical mycology
  • Biophotonics

Background:

  • Onychomycosis (fungal nail infection) treatment currently relies on antimycotic drugs.
  • Photodynamic therapy presents a potential alternative with a different mechanism of action.
  • Understanding photosensitizer-fungi interactions is crucial for developing effective photodynamic inactivation strategies.

Purpose of the Study:

  • To evaluate time-resolved 2D singlet oxygen luminescence detection and 2D fluorescence scanning.
  • To understand photosensitizer behavior when applied to fungi on Petri dishes.
  • To investigate photosensitizer-fungi interactions in various concentrations and growth stages.

Main Methods:

  • Utilized time-resolved 2D singlet oxygen luminescence detection and 2D fluorescence scanning.
  • Investigated two photosensitizers (cationic porphyrin, cationic corrole) and two fungi (Trichophyton rubrum, Scopulariopsis brevicaulis).
  • Monitored photosensitizer fluorescence and singlet oxygen luminescence over 24 days in darkness to avoid inactivation.

Main Results:

  • Two-dimensional correlation revealed differences in photosensitizer diffusion.
  • Singlet oxygen luminescence was quenched with increasing fungal growth.
  • Kinetics of singlet oxygen luminescence were detectable for both photosensitizers and fungi for up to seven days.

Conclusions:

  • The study provides a foundational understanding of photosensitizer-fungi interactions for photodynamic therapy.
  • Time-resolved luminescence detection is a viable tool for assessing photosensitizer behavior in this context.
  • Further development is needed to optimize photodynamic inactivation of onychomycosis pathogens.
Keywords:
OnychomycosisPhotodynamic inactivation of microorganismsPhotodynamic therapyScopulariopsis brevicaulisSinglet oxygenTrichophyton rubrum

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