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Updated: Feb 16, 2026

A Novel Method to Determine the Longitudinal Antibacterial Activity of Drug-Eluting Materials
Published on: March 3, 2023
Trixis angustifolia hexanic extract displays synergistic antibacterial activity against M. tuberculosis
Anahí C Sánchez-Chávez1, Anuar Salazar-Gómez2, L Gerardo Zepeda-Vallejo1
1a Departamento de Química Orgánica , Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional , Ciudad de México , México.
Abstract:
A phytochemical and antibacterial study of Trixis angustifolia, a species endemic to Mexico, was performed allowing the isolation of six flavones. The minimal inhibitory concentration (MIC) of the hexanic extract, against Mycobacterium tuberculosis H37Rv was 25 μg/mL. The hexanic extract caused a significant inhibition of intracellular mycobacterial growth at 12.5 μg/mL. The biodirected assay of hexane extract enabled the detection of an active fraction (AF) against M. tuberculosis (MIC = 12.5 μg/mL), and a major flavone 1 (pebrellin) with no antimycobacterial activity (MIC > 200 μg/mL). A subsequent combination antimicrobial assay showed a synergistic antimycobacterial effect of AF in combination with pebrellin; the results of the synergistic activity suggest that the antimycobacterial activity found in T. angustifolia is due to the combined action of diverse metabolites present in the plant.
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