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Updated: Feb 16, 2026

Human Placental and Decidual Organ Cultures to Study Infections at the Maternal-fetal Interface
Published on: July 21, 2016
Fetal-sex determination of human placental tissues
Séverine A Degrelle1, Thierry Fournier1
1INSERM, UMR-S1139, Faculté de Pharmacie De Paris, Paris F-75006, France; Université Paris Descartes, Sorbonne Paris Cité, Paris F-75006, France; Fondation PremUp, Paris, F-75006, France.
Abstract:
It is now demonstrated that the sex-specific maternal-placental-fetal interaction plays an important role in placental functions and pathologies. Determination of fetal-sex may therefore be an important consideration in studies using placenta samples. In this present study, we describe a simple, fast, and cheap protocol, which allows the fetal-sex determination of placental tissues from various starting materials (villi or formalin-fixed, paraffin-embedded (FFPE) tissues, isolated cytotrophoblasts or cellular debris from whole cell lysates, and cDNA) by a single duplex PCR reaction followed by agarose gel electrophoresis.
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