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Related Concept Videos

NMR Spectroscopy Of Amines01:19

NMR Spectroscopy Of Amines

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In proton NMR spectroscopy, primary amines and secondary amines showcase their N–H protons as a broad signal in the chemical shift range between δ 0.5 and 5 ppm. The exact position in this range depends on several factors, including sample concentration, hydrogen bonding, and the type of solvent used. Since amine protons undergo fast proton exchange in solution, the protons are labile and therefore do not participate in any splitting with adjacent protons. Thus, the observed peak is...
11.2K
NMR Spectroscopy of Aromatic Compounds01:14

NMR Spectroscopy of Aromatic Compounds

6.4K
Aromatic compounds can be identified or analyzed using proton NMR and carbon‐13 NMR. Typically, aromatic hydrogens or hydrogens directly bonded to the aromatic rings are strongly deshielded by the aromatic ring current. Therefore, they absorb in the range of 6.5–8.0 ppm in proton NMR spectra. For instance, aromatic hydrogens directly bonded to the benzene ring absorb at 7.3 ppm. However, aromatic hydrogens of larger rings absorb farther upfield or downfield than the ideal range.
6.4K
NMR Spectroscopy of Benzene Derivatives01:34

NMR Spectroscopy of Benzene Derivatives

11.4K
Simple unsubstituted benzene has six aromatic protons, all chemically equivalent. Therefore, benzene exhibits only a singlet peak at δ 7.3 ppm in the 1H NMR spectrum. The observed shift is far downfield because the aromatic ring current strongly deshields the protons. Any substitution on the benzene ring makes the aromatic protons nonequivalent, and the protons split each other. The peak is, therefore, no longer a singlet and the splitting pattern and their associated coupling...
11.4K
NMR Spectroscopy: Chemical Shift Overview01:15

NMR Spectroscopy: Chemical Shift Overview

3.4K
The position of the absorption signal of a sample is reported relative to the position of the signal of tetramethylsilane (TMS), which is added as an internal reference while recording spectra. The difference between the absorption frequencies of the sample and TMS (in Hz) is divided by the spectrometer operating frequency (in MHz) to obtain a dimensionless quantity called the chemical shift. It is reported on the δ (delta) scale and expressed in parts per million.
For instance, the proton...
3.4K
NMR Spectroscopy: Spin–Spin Coupling01:08

NMR Spectroscopy: Spin–Spin Coupling

3.3K
The spin state of an NMR-active nucleus can have a slight effect on its immediate electronic environment. This effect propagates through the intervening bonds and affects the electronic environments of NMR-active nuclei up to three bonds away; occasionally, even farther. This phenomenon is called spin–spin coupling or J-coupling. Coupling interactions are mutual and result in small changes in the absorption frequencies of both nuclei involved. While nuclei of the same element are involved...
3.3K
NMR and Mass Spectroscopy of Carboxylic Acids01:30

NMR and Mass Spectroscopy of Carboxylic Acids

5.3K
In ¹H NMR spectroscopy, acidic protons (–COOH) of carboxylic acids are highly deshielded and absorb far downfield, at around 9–12 ppm. The chemical shift value depends on the concentration and solvent used.
While α protons of carboxylic acids absorb at 2–2.5 ppm, β protons absorb further upfield.
Carboxylic acids are easily identified by dissolving them in deuterium oxide, which results in a rapid exchange of the acidic protons with deuterium. This leads to the...
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Updated: Feb 16, 2026

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
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Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR

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RNA Characterization by Solid-State NMR Spectroscopy.

Yufei Yang1, Shenlin Wang1

  • 1College of Chemistry and Molecular Engineering and Beijing NMR Center, Peking University, No.5 Yiheyuan Road, Haidian District, Beijing, 100871, P. R. China.

Chemistry (Weinheim an Der Bergstrasse, Germany)
|December 27, 2017
PubMed
Summary
This summary is machine-generated.

Magic-angle-spinning solid-state NMR (MAS SSNMR) offers a powerful method for determining RNA structures, crucial for understanding biological functions. This review highlights recent advances, techniques, and applications of MAS SSNMR in RNA structural characterization.

Keywords:
RNAfast magic-angle spinningsolid-state NMR spectroscopystructural investigationsstructure-function relationships

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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

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Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biophysics

Background:

  • RNA molecules are essential for numerous biological processes, and their structures are key to understanding their functions.
  • Determining the precise three-dimensional structure of RNA remains a significant challenge in molecular biology.
  • Magic-angle-spinning solid-state Nuclear Magnetic Resonance (MAS SSNMR) spectroscopy has emerged as a valuable technique for RNA structure elucidation.

Purpose of the Study:

  • To provide an overview of recent advancements in RNA structural characterization using MAS SSNMR.
  • To introduce effective sample preparation strategies and spectroscopic techniques for RNA structure determination.
  • To showcase successful applications of MAS SSNMR in investigating RNA molecules and complexes.

Main Methods:

  • Utilizing magic-angle-spinning solid-state NMR (MAS SSNMR) spectroscopy for atomic-level structural and dynamic analysis of RNA.
  • Implementing optimized sample preparation protocols tailored for solid-state NMR studies of RNA.
  • Applying various SSNMR spectroscopic techniques to identify and resolve RNA structural elements.

Main Results:

  • MAS SSNMR has demonstrated success in providing high-resolution structural information for various RNA molecules.
  • The technique has been effectively applied to study complex RNA-protein interactions.
  • Recent progress showcases the growing capability of MAS SSNMR in detailed RNA structural investigations.

Conclusions:

  • MAS SSNMR is a powerful and increasingly utilized technique for RNA structure determination.
  • Continued technical development in MAS SSNMR will further enhance its utility in structural biology.
  • This method provides critical insights into RNA structure-function relationships.