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Deregulation of sialidases in human normal and tumor tissues

Matilde Forcella1, Alessandra Mozzi1, Federico M Stefanini2

  • 1Department of Biotechnologies and Biosciences, University of Milano-Bicocca, Milan, Italy.

Cancer Biomarkers : Section a of Disease Markers
|December 28, 2017
PubMed
Abstract

Insights

Sialidase NEU3 promotes cancer progression, while NEU1 and NEU4 oppose it. This study found NEU1 and NEU3 are upregulated in most cancers, with NEU4 downregulated, offering potential diagnostic and therapeutic targets.

Area of Science:

  • Biochemistry
  • Oncology
  • Molecular Biology

Background:

  • Aberrant sialylation is a hallmark of cancer.
  • Mammalian sialidases (NEU1, NEU2, NEU3, NEU4) exhibit distinct roles in carcinogenesis.
  • NEU3 promotes cancer progression, whereas NEU1, NEU2, and NEU4 counteract malignant phenotypes.

Purpose of the Study:

  • To investigate the deregulation of NEU1, NEU3, and NEU4 transcripts in various cancer types.
  • To enhance understanding of sialidase involvement in cancer progression.
  • To analyze transcript levels in paired normal and tumor tissues from 170 patients across 11 cancer types.

Main Methods:

  • mRNA extraction from patient tissue specimens.
  • Retrotranscription of mRNA into cDNA.
  • Quantification of cDNA using Real-Time PCR.

Main Results:

  • NEU1 and NEU3 transcripts were upregulated in most cancer types studied.
  • NEU4 transcripts were downregulated in the majority of cancer types.
  • Colorectal and ovarian cancers showed particularly high upregulation of NEU3 and NEU1/NEU3, respectively.

Conclusions:

  • Human sialidase expression is significantly altered in tumors compared to healthy tissues.
  • Expression patterns of sialidases differ between European and Asian populations.
  • Sialidase deregulation presents potential applications in cancer diagnosis and therapy.
Keywords:
Sialomecancer progressiondiagnosis and therapysialidase expression

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