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Cytotoxic and the proliferative effect of cuttlefish bone on MC3T3-E1 osteoblast cell line

La-Ongthong Vajrabhaya1, Suwanna Korsuwannawong2, Rudee Surarit3

  • 1Endodontic Section, Faculty of Dental Medicine, Rangsit University, Pathumtanee 12000, Thailand.

European Journal of Dentistry
|December 28, 2017
PubMed
Abstract

Insights

Cuttlefish bone powder (CBP) did not harm osteoblast cells and significantly promoted their proliferation. CBP shows potential for enhancing osteoblast numbers in oral bone defects.

Area of Science:

  • Biomaterials Science
  • Cell Biology
  • Regenerative Medicine

Background:

  • Osteoblast proliferation is crucial for bone regeneration.
  • Cuttlefish bone is a potential source of biomaterials for bone tissue engineering.

Purpose of the Study:

  • To investigate the effects of cuttlefish bone powder (CBP) on MC3T3-E1 osteoblast cell line.
  • To evaluate the cytotoxic and proliferative effects of CBP on osteoblasts.

Main Methods:

  • MC3T3-E1 cells were exposed to varying concentrations of CBP (0.5–200 μg/ml).
  • Cytotoxicity was assessed using the MTT assay.
  • Cell proliferation was measured over 16 days using the MTT assay.

Main Results:

  • CBP exhibited no cytotoxic effects on MC3T3-E1 cells across all tested concentrations.
  • Cell viability showed a non-significant, dose-dependent decrease.
  • Significant osteoblast proliferation was observed at 16 days with CBP treatment (0.5, 25, and 100 μg/ml).

Conclusions:

  • Cuttlefish bone powder (CBP) is non-cytotoxic to osteoblasts.
  • CBP significantly enhances osteoblast proliferation.
  • CBP holds promise as a biomaterial for augmenting osteoblast populations in oral bone defects.
Keywords:
Cuttlefish bone powdercytotoxicityosteoblast cell cultureproliferation

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