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TSR2 Induces laryngeal cancer cell apoptosis through inhibiting NF-κB signaling pathway

Hong-Jiang He1, Han Bing2, Guijun Liu3

  • 1Department of Head and Neck Surgery, Affiliated Tumor Hospital of Harbin Medical University, Harbin, China.

The Laryngoscope
|December 28, 2017
PubMed
Abstract

Insights

TSR2 is downregulated in laryngeal squamous cell carcinoma (LSCC). Overexpressing TSR2 promotes LSCC cell apoptosis by inhibiting the NF-κB signaling pathway, offering a potential gene therapy target.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Human laryngeal squamous cell carcinoma (LSCC) is a malignancy originating in the laryngeal mucosa epithelium.
  • The precise molecular mechanisms driving LSCC progression remain unclear.
  • Investigating novel molecular targets is crucial for developing effective LSCC therapies.

Purpose of the Study:

  • To elucidate the role of TSR2 in LSCC.
  • To investigate the molecular mechanisms by which TSR2 influences LSCC cell apoptosis.
  • To explore TSR2 as a potential therapeutic target for LSCC.

Main Methods:

  • Quantitative real-time polymerase chain reaction (qRT-PCR) to assess TSR2 expression in LSCC tissues and cells.
  • Overexpression of TSR2 in LSCC cell lines (Hep-2 and AMC-HN-8) using pcDNA3.1-TSR2.
  • Western blot and terminal dUTP nick end-labeling assays to evaluate apoptosis-related proteins and NF-κB p65 nuclear translocation.

Main Results:

  • TSR2 was found to be significantly downregulated in LSCC tissues and cells compared to normal controls.
  • Overexpression of TSR2 promoted apoptosis in LSCC cells and upregulated apoptosis-related proteins.
  • TSR2 overexpression inhibited the NF-κB signaling pathway by reducing nuclear NF-κB p65 and increasing cytoplasmic NF-κB p65, while also inhibiting IκBα and IKKα/β phosphorylation.

Conclusions:

  • TSR2 plays a suppressive role in LSCC development.
  • TSR2-induced apoptosis in LSCC is mediated through the inhibition of the NF-κB signaling pathway.
  • TSR2 represents a promising molecular target for gene therapy in LSCC treatment.
Keywords:
Human laryngeal squamous cell carcinomaNF-κBTSR2cell apoptosis

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