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p58, a Cytoskeletal Protein, Is Associated With Muscle Cell Determinants in Ascidian Eggs.

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Related Experiment Video

Updated: Feb 16, 2026

Direct Delivery of MIF Morpholinos Into the Zebrafish Otocyst by Injection and Electroporation Affects Inner Ear Development
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Direct Development in the Ascidian Molgula retortiformis (Verrill, 1871).

W R Bates

    The Biological Bulletin
    |December 28, 2017
    PubMed
    Summary

    Directly developing ascidians like Molgula retortiformis lack muscle cells at hatching. This may be due to a missing factor in the egg cytoplasm, impacting muscle development and evolution.

    Area of Science:

    • Developmental Biology
    • Evolutionary Biology
    • Cellular Biology

    Background:

    • Ascidians exhibit diverse developmental strategies, including direct and indirect development.
    • Understanding these differences aids in comprehending the evolution of developmental processes.
    • Molgula retortiformis is a direct-developing ascidian species suitable for such studies.

    Purpose of the Study:

    • To investigate the cellular features of the direct-developing ascidian Molgula retortiformis.
    • To compare developmental processes between direct and indirect developing ascidians.
    • To explore the reasons behind the absence of muscle cells in newly hatched M. retortiformis.

    Main Methods:

    • Transmission electron microscopy
    • Histochemistry

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  • Immunocytochemistry using a vertebrate intermediate filament antibody (NN18)
  • Main Results:

    • M. retortiformis eggs contain abundant yolk and glycogen.
    • Newly hatched juveniles show similar cell ultrastructure, with deeper cells richer in yolk and glycogen.
    • Differentiated muscle cells and acetylcholinesterase (AChE) activity were absent in hatchlings.

    Conclusions:

    • The absence of muscle cells in M. retortiformis juveniles may stem from a lack of a specific factor in the egg's myoplasm.
    • This finding supports the "substrate hypothesis" regarding ascidian direct development.
    • Further research can elucidate the evolution of developmental patterns in ascidians.