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Updated: Feb 16, 2026

Reconstitution of Membrane-Tethered Minimal Actin Cortices on Supported Lipid Bilayers
Published on: July 12, 2022
Promoting Multivalent Antibody-Antigen Interactions by Tethering Antibody Molecules on a PEGylated
Po-Ying Yeh1,2, Yih-Ruey Chen1, Chien-Fang Wang1
1Genomics Research Center, Academia Sinica , 128, Sec 2, Academic Road, Nankang, Taipei 115, Taiwan.
Abstract:
To efficiently isolate maximal quantity of circulating tumor cells (CTCs) and circulating tumor cell microembolis (CTMs) from patient blood by antibody coated microfluidics, a multifunctional, pegylated polyamidoamine-dendrimers conjugated supported lipid bilayer surface construct was proposed to enhance accessibility of antibody molecules to the antigen molecules on target CTCs. The combination of a hydrated, stretchable dendrimer and a laterally mobile supported lipid bilayer (SLB) provide attached antibody molecules with 2.5-dimensional chain movement, achieving multivalency between the surface antibody and cell antigen molecules. An over 170% enhancement is distinctive for Panc-1 cells that expresses low antigen level. Of seven pancreatic ductal adenocarcinoma patients, an average 440 single CTCs and 90 CTMs were collected in 2 mL of peripheral blood, which were 1.6 times and 2.3 times more, than those captured by the SLB-only microfluidics. In summary, we have demonstrated a material design to enhance multivalent antibody-antigen interaction, which is useful for rare cell enrichment and cancer detection.
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