Boronate affinity solid-phase extraction of cis-diol compounds by a one-step electrochemically synthesized selective
Xu Ling1,2, Zilin Chen3,4
1Key Laboratory of Combinatorial Biosynthesis and Drug Discovery (Wuhan University), Ministry of Education, and Wuhan University School of Pharmaceutical Science, Wuhan, Hubei, 430071, China.
Analytical and Bioanalytical Chemistry
|December 29, 2017
Summary
A novel conductive sorbent, poly(thiophene-3-boronic acid), enhances boronate affinity extraction for cis-diol compounds. This method offers high efficiency and sensitivity for analyzing catecholamines in biological samples.
Area of Science:
- Analytical Chemistry
- Materials Science
- Biochemistry
Background:
- Boronate affinity extraction is crucial for isolating cis-diol compounds.
- Developing efficient and selective sorbents is essential for analytical applications.
- Catecholamines are important biomarkers in biological fluids.
Purpose of the Study:
- To develop a rational-designed conductive sorbent for boronate affinity extraction.
- To apply the sorbent in an online solid-phase microextraction-high-performance liquid chromatography system.
- To analyze catecholamines in biological samples with high efficiency and sensitivity.
Main Methods:
- Electrochemical deposition of poly(thiophene-3-boronic acid) on a carbon fiber bundle.
- Packing the coated fiber bundle into a poly(ether ether ketone) tube for online solid-phase microextraction.
- High-performance liquid chromatography analysis of catecholamines (adrenaline, noradrenaline, dopamine).
Main Results:
- Achieved over 600-fold extraction efficiency for cis-diol compounds.
- Obtained low limits of detection (0.1-0.2 ng·mL⁻¹).
- Demonstrated high recoveries (92.5-95.71%) in spiked plasma samples.
Conclusions:
- The electrochemically synthesized sorbent shows high specificity and extraction efficiency.
- The developed method is suitable for the analysis of catecholamines in biological matrices.
- This sorbent holds significant potential for research involving cis-diol compounds.
Keywords:
Boronate affinityCarbon fiber bundlesCatecholaminesConductive polymerSolid-phase microextractionMore Related Videos
08:41Integration of Miniaturized Solid Phase Extraction and LC-MS/MS Detection of 3-Nitrotyrosine in Human Urine for Clinical Applications
Published on: July 14, 2017
10.0K
13:42Solid-phase Submonomer Synthesis of Peptoid Polymers and their Self-Assembly into Highly-Ordered Nanosheets
Published on: November 2, 2011
30.1K
Related Concept Videos
02:34Polymers
41.5K
The word polymer is derived from the Greek words “poly” which means “many” and “mer” which means “parts”. Polymers are long chains of molecules composed of repeating units of smaller molecules, known as monomers. They either occur naturally, such as DNA and proteins, or can be constructed synthetically, like plastics. They have varied structural characteristics, such as linear chains, branched chains, or complex networks, that contribute to the...
41.5K
02:02Cis-regulatory Sequences
11.9K
Cis-regulatory sequences are short fragments of non-coding DNA that are present on the same chromosomes as the genes that they regulate. These fragments serve as binding sites for transcriptional regulators, proteins that are responsible for controlling gene transcription and differential gene expression across cell types in eukaryotes. Cis-regulatory sequences can be close to the gene of interest or thousands of bases away in the DNA sequence; however, those sequences that are further away are...
11.9K
01:20Types of Step-Growth Polymers: Polyesters
2.6K
The introduction of polyesters has brought major development to the textile industry. The wrinkle-free behavior of polyester blends has eliminated the need for starching and ironing clothes.
Polyesters are commonly prepared from terephthalic acid and ethylene glycol; the crude product is known as poly(ethylene terephthalate) or PET. However, polyesters are synthesized industrially by transesterification of dimethyl terephthalate with ethylene glycol at 150 °C. The two reactants and the polymer...
Polyesters are commonly prepared from terephthalic acid and ethylene glycol; the crude product is known as poly(ethylene terephthalate) or PET. However, polyesters are synthesized industrially by transesterification of dimethyl terephthalate with ethylene glycol at 150 °C. The two reactants and the polymer...
2.6K
01:08Molecular Weight of Step-Growth Polymers
2.9K
Step growth polymerization involves bi or multifunctional monomers. Bifunctional monomers react to form linear step growth polymers, whereas multifunctional monomers react to form non-linear or branched polymers.
As the step-growth polymerization involves step-wise condensation of monomers, the molecular weight also builds up eventually. Consequently, high molecular weight polymers are obtained at the late stages of the polymerization, where 99% of monomers have been consumed.
The extent of the...
As the step-growth polymerization involves step-wise condensation of monomers, the molecular weight also builds up eventually. Consequently, high molecular weight polymers are obtained at the late stages of the polymerization, where 99% of monomers have been consumed.
The extent of the...
2.9K
01:26What is an Electrochemical Gradient?
128.7K
Adenosine triphosphate, or ATP, is considered the primary energy source in cells. However, energy can also be stored in the electrochemical gradient of an ion across the plasma membrane, which is determined by two factors: its chemical and electrical gradients.
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
The chemical gradient relies on differences in the abundance of a substance on the outside versus the inside of a cell and flows from areas of high to low ion concentration. In contrast, the electrical gradient revolves around an...
128.7K
03:07Electron Affinity
43.8K
The electron affinity (EA) is the energy change for adding an electron to a gaseous atom to form an anion (negative ion).
43.8K