Related Experiment Video
Updated: Feb 16, 2026
10:30
Visualization of IL-22-expressing Lymphocytes Using Reporter Mice
Published on: January 25, 2017
9.3K
Expression analyses of Dusp22 (Dual-specificity phosphatase 22) in mouse tissues
Nanako Hamada1, Makoto Mizuno1, Hiroyuki Tomita2
1Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Human Service Center, 713-8 Kamiya, Kasugai, 480-0392, Japan.
Medical Molecular Morphology
|December 29, 2017
Summary
Researchers developed an anti-Dusp22 antibody to study dual-specificity phosphatase 22 (Dusp22) expression and localization. The antibody confirmed Dusp22
Area of Science:
- Biochemistry
- Cell Biology
- Molecular Biology
Background:
- Dual-specificity phosphatase 22 (Dusp22) regulates cellular functions via protein dephosphorylation.
- Understanding Dusp22 expression and localization is crucial for elucidating its biological roles.
Purpose of the Study:
- To generate a specific antibody against Dusp22 (anti-Dusp22).
- To analyze the expression profile and cellular localization of Dusp22 in mouse tissues and cell lines.
Main Methods:
- Western blotting for expression analysis in mouse tissues and cell lines.
- Immunofluorescence microscopy to determine subcellular localization in NIH3T3 cells.
- Generation and validation of a specific anti-Dusp22 antibody.
Main Results:
- Anti-Dusp22 antibody successfully detected Dusp22 in various mouse tissues and cell lines.
- Dusp22 exhibited a tissue-dependent expression pattern in adult mice.
- Evidence suggested the existence of larger molecular mass isoforms of Dusp22.
- In NIH3T3 cells, Dusp22 localized to the cytoplasm and partially colocalized with the actin cytoskeleton.
Conclusions:
- The developed anti-Dusp22 antibody is a valuable tool for biochemical and cell biological studies of Dusp22.
- Dusp22 expression is regulated in a tissue-specific manner.
- Dusp22 interacts with the actin cytoskeleton, suggesting a role in cytoskeletal regulation.
Related Concept Videos
02:54Protein Kinases and Phosphatases
15.2K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
15.2K
02:54Protein Kinases and Phosphatases
4.5K
4.5K
01:58Cell Specific Gene Expression
16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
01:58Cell Specific Gene Expression
5.6K
5.6K
02:35Chromatin Position Affects Gene Expression
25.0K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
25.0K
01:42What is Gene Expression?
197.2K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
197.2K