Related Experiment Video

Updated: Feb 16, 2026

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers
14:03

High-Throughput Cardiotoxicity Screening Using Mature Human Induced Pluripotent Stem Cell-Derived Cardiomyocyte Monolayers

Published on: March 24, 2023

2.5K

Creatine phosphate disodium salt protects against Dox-induced cardiotoxicity by increasing calumenin

Yu Wang1,2, Ying Sun3, Xin Guo1,2

  • 1Inner Mongolia University for the Nationalities, No. 22 Holin He Street, Tongliao, 028002, Inner Mongolia, People's Republic of China.

Medical Molecular Morphology
|December 29, 2017
PubMed

Insights

Creatine phosphate disodium salt (CP) protects heart cells from injury by reducing endoplasmic reticulum stress (ERS)-induced apoptosis. This cardioprotective effect may involve CP increasing calumenin levels.

Area of Science:

  • Cardiovascular Biology
  • Cellular Stress Response
  • Pharmacology

Background:

  • Endoplasmic reticulum stress (ERS)-induced apoptosis is a potential therapeutic target for cardiovascular diseases.
  • Creatine phosphate disodium salt (CP) exhibits cardioprotective effects, but its impact on ERS is not well understood.

Purpose of the Study:

  • To elucidate the mechanism of CP's cardioprotection against doxorubicin (Dox)-induced cardiomyocyte injury.
  • To investigate the role of ERS and calumenin in CP's protective effects.

Main Methods:

  • Neonatal rat cardiomyocytes (NRC) were subjected to Dox-induced injury and treated with CP.
  • Cell viability, apoptosis, and expression of ERS markers (grp78, grp94, calumenin, C/EBP homologous protein) were assessed.
  • The function of calumenin was evaluated by down-regulating its expression.

Main Results:

  • CP treatment reduced Dox-induced apoptosis and alleviated ERS in NRC.
  • CP treatment led to an increase in calumenin expression under Dox-induced stress.
  • CP down-regulated the ERS effector C/EBP homologous protein, an effect influenced by calumenin levels.

Conclusions:

  • CP exerts cardioprotection by inhibiting ERS-induced apoptosis in cardiomyocytes.
  • The mechanism of CP's protective effect may be associated with its ability to increase calumenin expression.
Keywords:
Creatine phosphate disodium saltDoxorubicinEndoplasmic reticulum stress

More Related Videos

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification10:05

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Published on: May 6, 2018

13.4K
A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model05:14

A Calcium Phosphate-Induced Mouse Abdominal Aortic Aneurysm Model

Published on: November 18, 2022

4.5K

Related Concept Videos

Responses to Salt Stress02:02

Responses to Salt Stress

Salt stress—which can be triggered by high salt concentrations in a plant’s environment—can significantly affect plant growth and crop production by influencing photosynthesis and the absorption of water and nutrients.
14.7K
Determining the pH of Salt Solutions04:08

Determining the pH of Salt Solutions

The pH of a salt solution is determined by its component anions and cations. Salts that contain pH-neutral anions and the hydronium ion-producing cations form a solution with a pH less than 7. For example, in ammonium nitrate (NH4NO3) solution, NO3− ions do not react with water whereas NH4+ ions produce the hydronium ions resulting in the acidic solution.  In contrast, salts that contain pH-neutral cations and the hydroxide ion-producing anions form a solution with a pH greater than 7. For...
48.3K
Phosphate Buffer01:22

Phosphate Buffer

The phosphate buffer system is a critical biological mechanism for maintaining pH stability in the body. This system operates primarily through two components: sodium dihydrogen phosphate (NaH2PO4), which acts as a weak acid, and sodium hydrogen phosphate (Na2HPO4), which serves as a weak base.
Sodium dihydrogen phosphate does not fully dissociate in neutral or acidic solutions. When a strong base, such as sodium hydroxide (NaOH), is introduced into the solution, sodium dihydrogen phosphate...
5.4K
Increasing Function01:18

Increasing Function

An increasing function exhibits a rise in output values as input values increase. This behavior is depicted graphically as a curve or line that slopes upward from left to right. Such a function satisfies the condition that if x1 < x2, then f(x1) < f(x2), indicating that the function values grow with increasing inputs. This concept is fundamental in understanding growth trends across various domains, such as population dynamics, financial investments, or resource consumption.The...
403
Protection of Alcohols02:31

Protection of Alcohols

This lesson delves into the concept of protection and deprotection of a functional group fundamental to synthetic organic chemistry. These phenomena are explained in the context of aliphatic and aromatic alcohols.
Protection
It defines a protecting group as the masking agent to make the more reactive species inert to a given set of conditions. This concept is depicted via the illustration of liquid flow through different outlets in an assembly of pipes. The analogy helps to understand the role...
8.1K
Zones of Protection01:16

Zones of Protection

In power systems, the entire setup is divided into protective zones to isolate faults and protect the rest of the network. These zones include generators, transformers, buses, transmission lines, distribution lines, and motors. Each zone can be visualized as a separate room in a house, with each room protected by its own circuit breaker.
Protective zones are defined by closed dashed lines, containing one or more components. A key characteristic of these zones is the strategic placement of...
821