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Development of a Polymicrobial Colony Biofilm Model to Test Antimicrobials in Cystic Fibrosis
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Species distribution, sequence types and antimicrobial resistance of Acinetobacter spp. from cystic fibrosis patients

G A Rocha1, D F Lima1, E R Rodrigues1

  • 1Departamento de Microbiologia,Imunologia e Parasitologia,Universidade do Estado do Rio de Janeiro,Rio de Janeiro, RJ,Brazil.

Epidemiology and Infection
|December 29, 2017
PubMed

Insights

Acinetobacter species were identified in cystic fibrosis (CF) patients, revealing significant diversity and multidrug resistance. This highlights challenges in treating CF lung infections with these opportunistic pathogens.

Area of Science:

  • Medical Microbiology
  • Infectious Diseases
  • Genomics

Background:

  • Acinetobacter species are significant healthcare-associated pathogens.
  • These bacteria are increasingly linked to antibiotic resistance and global outbreaks.
  • Their presence in cystic fibrosis (CF) patients is uncommon.

Purpose of the Study:

  • To characterize Acinetobacter species found in Brazilian cystic fibrosis patients.
  • To investigate the antimicrobial resistance profiles and genetic diversity of these isolates.

Main Methods:

  • Isolation and identification of Acinetobacter species from patient sputum samples.
  • Partial rpoB gene sequencing for species identification.
  • Multidrug resistance (MDR) assessment using disk diffusion tests.
  • Multilocus sequence typing (MLST) for genotypic diversity analysis.

Main Results:

  • Fifty-three Acinetobacter strains were identified from 39 CF patients, representing diverse species including A. baumannii, A. pittii, and A. nosocomialis.
  • Sixteen strains (30%) exhibited multidrug resistance (MDR).
  • Eight MDR carbapenem-resistant A. baumannii strains carried the bla OXA-23-like gene.
  • Thirty-three distinct sequence types (STs) were identified, with eight novel STs and several STs found in multiple patients, indicating potential transmission.

Conclusions:

  • This study reveals substantial species diversity of Acinetobacter in CF lung infections.
  • The high prevalence of antimicrobial resistance, including carbapenem resistance, poses significant therapeutic challenges.
  • Further research is needed to understand the clinical implications and transmission dynamics of Acinetobacter in CF patients.
Keywords:
Acinetobacter spp.cystic fibrosismultidrug resistancemultilocus sequence typing

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