Related Experiment Video

Updated: Feb 16, 2026

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis
06:03

Oropharyngeal Administration of Bleomycin in the Murine Model of Pulmonary Fibrosis

Published on: May 9, 2025

1.9K

A Lipidomics Approach to Identifying Key Lipid Species Involved in VEGF-Inhibitor Mediated Attenuation of

Yogesh M Kulkarni1, Sucharita Dutta2, Anand Krishnan V Iyer1

  • 1Department of Pharmaceutical Sciences, School of Pharmacy, Hampton University, Hampton, VA, USA.

Proteomics. Clinical Applications
|December 29, 2017
PubMed
Abstract

Insights

Idiopathic pulmonary fibrosis (IPF) research reveals that lipid metabolism is key. Targeting lipid imbalance with CBO-P11 in a mouse model reduced fibrosis, suggesting new therapeutic avenues for IPF.

Area of Science:

  • Pulmonary Medicine
  • Lipidomics
  • Pathogenesis Research

Background:

  • Idiopathic pulmonary fibrosis (IPF) lacks effective therapies due to poor molecular understanding.
  • Impaired lipid metabolism and resulting lipid imbalance are understudied contributors to IPF pathogenesis.

Purpose of the Study:

  • To identify specific lipids involved in pulmonary fibrosis pathogenesis.
  • To investigate the therapeutic potential of targeting lipid metabolism in IPF.

Main Methods:

  • Utilized shotgun lipidomics in a bleomycin (BLM)-induced mouse model of pulmonary fibrosis.
  • Administered vascular endothelial growth factor (VEGF)-inhibitor CBO-P11 as a therapeutic intervention.

Main Results:

  • CBO-P11 treatment attenuated BLM-induced fibrotic responses.
  • Therapeutic intervention led to decreased total lipid content.
  • Specific lipids upregulated by BLM were downregulated with CBO-P11 cotreatment.

Conclusions:

  • Dysregulated lipids identified in this study are potential future biomarkers for IPF.
  • Targeting lipid metabolism presents a promising therapeutic strategy for IPF.
Keywords:
VEGF-inhibitorbleomyciningenuity pathway analysislipidomicspulmonary fibrosis

More Related Videos

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization02:46

A Mouse Model of Pulmonary Fibrosis Induced by Nasal Bleomycin Nebulization

Published on: January 20, 2023

4.9K
Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo06:29

Adoptive Transfer of IL-33-Stimulated Macrophages into Bleomycin-Induced Mouse Models to Study Their Effect on Idiopathic Pulmonary Fibrosis In Vivo

Published on: May 5, 2023

3.1K

Related Concept Videos

Transcription Attenuation in Prokaryotes02:42

Transcription Attenuation in Prokaryotes

Transcriptional attenuation occurs when RNA transcription is prematurely terminated due to the formation of a terminator mRNA hairpin structure.  Bacteria use these hairpins to regulate the transcription process and control the synthesis of several amino acids including histidine, lysine, threonine, and phenylalanine. Transcription attenuation takes place in the non-coding regions of mRNA.
There are several different mechanisms used to attenuate transcription. In ribosome mediated...
18.6K
Treatment for Pulmonary Arterial Hypertension: Phosphodiesterase Inhibitors01:28

Treatment for Pulmonary Arterial Hypertension: Phosphodiesterase Inhibitors

Phosphodiesterase 5 (PDE5) inhibitors are potent enzymes that function to hydrolyze cyclic nucleotides to their corresponding 5' monophosphates. Their unique biochemical properties have been applied in treating Pulmonary Arterial Hypertension (PAH).
Among the PDE5 inhibitors, sildenafil (Revatio) stands out as a competitive and selective inhibitor. It operates by elevating cellular levels of cGMP and augmenting signaling through the cGMP-PKG pathway, promoting vasodilation. Upon oral...
630
What is a Species?01:17

What is a Species?

Overview
50.6K
Keystone Species01:39

Keystone Species

Measures of species biodiversity, such as richness (i.e., the number of species present) and evenness (i.e., their relative abundance), describe an ecological community’s structure. Many factors affect community structure, including abiotic factors (e.g., sunlight and nutrients), disturbances (e.g., fire or flood), species interactions (e.g., predation or competition), and chance events (e.g., foreign species invasion). Certain species—such as keystone species—also play a...
25.1K
Structure of Lipids03:38

Structure of Lipids

Lipids include a diverse group of compounds that are largely nonpolar in nature. This is because they are hydrocarbons that include mostly nonpolar carbon-carbon or carbon-hydrogen bonds. Non-polar molecules are hydrophobic (“water fearing”), or insoluble in water. Lipids perform many different functions in a cell. Cells store energy for long-term use in the form of fats. Lipids also provide insulation from the environment for plants and animals. For example, they help keep aquatic...
99.5K
Formation of Species01:31

Formation of Species

Speciation describes the formation of one or more new species from one or sometimes multiple original species. The resulting species are discrete from the parent species, and barriers to reproduction will typically exist. There are two primary mechanisms, speciation with and without geographic isolation—allopatric and sympatric speciation, respectively.
45.5K